FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3189118832> ?p ?o ?g. }

• W3189118832 endingPage "549" @default.
• W3189118832 startingPage "549" @default.
• W3189118832 abstract "Bites from helodermatid lizards can cause pain, paresthesia, paralysis, and tachycardia, as well as other symptoms consistent with neurotoxicity. Furthermore, in vitro studies have shown that Heloderma horridum venom inhibits ion flux and blocks the electrical stimulation of skeletal muscles. Helodermatids have long been considered the only venomous lizards, but a large body of robust evidence has demonstrated venom to be a basal trait of Anguimorpha. This clade includes varanid lizards, whose bites have been reported to cause anticoagulation, pain, and occasionally paralysis and tachycardia. Despite the evolutionary novelty of these lizard venoms, their neuromuscular targets have yet to be identified, even for the iconic helodermatid lizards. Therefore, to fill this knowledge gap, the venoms of three Heloderma species (H. exasperatum, H. horridum and H. suspectum) and two Varanus species (V. salvadorii and V. varius) were investigated using Gallus gallus chick biventer cervicis nerve-muscle preparations and biolayer interferometry assays for binding to mammalian ion channels. Incubation with Heloderma venoms caused the reduction in nerve-mediated muscle twitches post initial response of avian skeletal muscle tissue preparation assays suggesting voltage-gated sodium (NaV) channel binding. Congruent with the flaccid paralysis inducing blockage of electrical stimulation in the skeletal muscle preparations, the biolayer interferometry tests with Heloderma suspectum venom revealed binding to the S3-S4 loop within voltage-sensing domain IV of the skeletal muscle channel subtype, NaV1.4. Consistent with tachycardia reported in clinical cases, the venom also bound to voltage-sensing domain IV of the cardiac smooth muscle calcium channel, CaV1.2. While Varanus varius venom did not have discernable effects in the avian tissue preparation assay at the concentration tested, in the biointerferometry assay both V. varius and V. salvadorii bound to voltage-sensing domain IV of both NaV1.4 and CaV1.2, similar to H. suspectum venom. The ability of varanid venoms to bind to mammalian ion channels but not to the avian tissue preparation suggests prey-selective actions, as did the differential potency within the Heloderma venoms for avian versus mammalian pathophysiological targets. This study thus presents the detailed characterization of Heloderma venom ion channel neurotoxicity and offers the first evidence of varanid lizard venom neurotoxicity. In addition, the data not only provide information useful to understanding the clinical effects produced by envenomations, but also reveal their utility as physiological probes, and underscore the potential utility of neglected venomous lineages in the drug design and development pipeline." @default.
• W3189118832 created "2021-08-16" @default.
• W3189118832 creator A5003358124 @default.
• W3189118832 creator A5010141734 @default.
• W3189118832 creator A5012209500 @default.
• W3189118832 creator A5014958666 @default.
• W3189118832 creator A5025065239 @default.
• W3189118832 creator A5027586688 @default.
• W3189118832 creator A5051021501 @default.
• W3189118832 creator A5073667848 @default.
• W3189118832 creator A5079574192 @default.
• W3189118832 date "2021-08-06" @default.
• W3189118832 modified "2023-09-26" @default.
• W3189118832 title "The Dragon’s Paralysing Spell: Evidence of Sodium and Calcium Ion Channel Binding Neurotoxins in Helodermatid and Varanid Lizard Venoms" @default.
• W3189118832 cites W13871547 @default.
• W3189118832 cites W1589976671 @default.
• W3189118832 cites W1974811012 @default.
• W3189118832 cites W1979228161 @default.
• W3189118832 cites W1980714687 @default.
• W3189118832 cites W1981733318 @default.
• W3189118832 cites W1998242611 @default.
• W3189118832 cites W2002155775 @default.
• W3189118832 cites W2006284876 @default.
• W3189118832 cites W2010362551 @default.
• W3189118832 cites W2021448182 @default.
• W3189118832 cites W2023435849 @default.
• W3189118832 cites W2025595983 @default.
• W3189118832 cites W2045678903 @default.
• W3189118832 cites W2047156991 @default.
• W3189118832 cites W2050472057 @default.
• W3189118832 cites W2069160688 @default.
• W3189118832 cites W2073492913 @default.
• W3189118832 cites W2076955847 @default.
• W3189118832 cites W2081273353 @default.
• W3189118832 cites W2082398160 @default.
• W3189118832 cites W2084829080 @default.
• W3189118832 cites W2089864996 @default.
• W3189118832 cites W2090919316 @default.
• W3189118832 cites W2095581992 @default.
• W3189118832 cites W2096701787 @default.
• W3189118832 cites W2100505309 @default.
• W3189118832 cites W2102681667 @default.
• W3189118832 cites W2106449364 @default.
• W3189118832 cites W2110509020 @default.
• W3189118832 cites W2118129389 @default.
• W3189118832 cites W2122467599 @default.
• W3189118832 cites W2126032630 @default.
• W3189118832 cites W2127494940 @default.
• W3189118832 cites W2128741370 @default.
• W3189118832 cites W2150774183 @default.
• W3189118832 cites W2158426869 @default.
• W3189118832 cites W2159332152 @default.
• W3189118832 cites W2163151214 @default.
• W3189118832 cites W2163255992 @default.
• W3189118832 cites W2166647530 @default.
• W3189118832 cites W2170154282 @default.
• W3189118832 cites W2197214562 @default.
• W3189118832 cites W2319743501 @default.
• W3189118832 cites W2536507227 @default.
• W3189118832 cites W2608810826 @default.
• W3189118832 cites W2728063140 @default.
• W3189118832 cites W2742809887 @default.
• W3189118832 cites W2767028951 @default.
• W3189118832 cites W2770462389 @default.
• W3189118832 cites W2886429358 @default.
• W3189118832 cites W2902077753 @default.
• W3189118832 cites W2912991414 @default.
• W3189118832 cites W2943955331 @default.
• W3189118832 cites W2966002566 @default.
• W3189118832 cites W2980670669 @default.
• W3189118832 cites W3013462984 @default.
• W3189118832 cites W3015272755 @default.
• W3189118832 cites W3024590918 @default.
• W3189118832 cites W3047710852 @default.
• W3189118832 cites W3080815165 @default.
• W3189118832 cites W3092502269 @default.
• W3189118832 cites W3095808780 @default.
• W3189118832 cites W3109109438 @default.
• W3189118832 cites W3118589946 @default.
• W3189118832 cites W3119613646 @default.
• W3189118832 cites W3127777167 @default.
• W3189118832 cites W3137681057 @default.
• W3189118832 cites W3154906376 @default.
• W3189118832 cites W4233294700 @default.
• W3189118832 doi "https://doi.org/10.3390/toxins13080549" @default.
• W3189118832 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8402328" @default.
• W3189118832 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34437420" @default.
• W3189118832 hasPublicationYear "2021" @default.
• W3189118832 type Work @default.
• W3189118832 sameAs 3189118832 @default.
• W3189118832 citedByCount "2" @default.
• W3189118832 countsByYear W31891188322021 @default.
• W3189118832 countsByYear W31891188322023 @default.
• W3189118832 crossrefType "journal-article" @default.
• W3189118832 hasAuthorship W3189118832A5003358124 @default.
• W3189118832 hasAuthorship W3189118832A5010141734 @default.
• W3189118832 hasAuthorship W3189118832A5012209500 @default.
• W3189118832 hasAuthorship W3189118832A5014958666 @default.

• next