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• W3189143106 abstract "The genetic encoding of artificial enzymes represents a substantial advantage relative to traditional molecular catalyst optimization, as laboratory-based directed evolution coupled with high-throughput screening methods can provide rapid development and functional characterization of enzyme libraries. However, these techniques have been of limited utility in the field of artificial metalloenzymes due to the need for in vitro cofactor metalation. Here, we report the development of methodology for in vivo production of nickel-substituted rubredoxin, an artificial metalloenzyme that is a structural, functional, and mechanistic mimic of the [NiFe] hydrogenases. Direct voltammetry on cell lysate establishes precedent for the development of an electrochemical screen. This technique will be broadly applicable to the in vivo generation of artificial metalloenzymes that require a non-native metal cofactor, offering a route for rapid enzyme optimization and setting the stage for integration of artificial metalloenzymes into biochemical pathways within diverse hosts." @default.
• W3189143106 created "2021-08-16" @default.
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• W3189143106 date "2021-08-09" @default.
• W3189143106 modified "2023-09-27" @default.
• W3189143106 title "<i>In Vivo</i> Assembly of a Genetically Encoded Artificial Metalloenzyme for Hydrogen Production" @default.
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• W3189143106 doi "https://doi.org/10.1021/acssynbio.1c00177" @default.
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