Matches in SemOpenAlex for { <https://semopenalex.org/work/W3189656476> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W3189656476 abstract "Polysome profile analysis is a popular method for separating polysomes and ribosomal subunits and is typically achieved using a sucrose density gradient (SDG). This has remained the gold standard method since ribosomes were first discovered; however, this method is time-consuming and requires multiple steps from making the gradient and long ultracentrifugation to collecting and analyzing the fractions. Each of these steps in the SDG workflow can introduce potential technical variation that affects the reproducibility of gradient profiles between samples. To address these limitations, we have developed a flexible, alternative approach for analyzing polysomes and ribosomal subunits based on size-exclusion chromatography (SEC), termed 'Ribo Mega-SEC.' In comparison with the SDG method, Ribo Mega-SEC involves a single step using ultra-high-performance liquid chromatography (uHPLC). The entire workflow, from injecting the lysate to collecting the fractions, can be performed in as little as 15 min, with high reproducibility. By varying the pore size of the SEC column, polysomes and ribosomal subunits can be separated using extracts from either human or mouse cultured cell lines or from tissue samples, Drosophila embryos, or budding yeast. The resulting separated fractions are suitable for analysis using a wide range of subsequent analytical techniques including mass spectrometry (MS)-based proteomics, RNA-Seq, electron microscopy (EM), and multiple biochemical assays." @default.
- W3189656476 created "2021-08-16" @default.
- W3189656476 creator A5014112981 @default.
- W3189656476 creator A5019806968 @default.
- W3189656476 creator A5025927634 @default.
- W3189656476 creator A5032278821 @default.
- W3189656476 creator A5076477268 @default.
- W3189656476 date "2021-01-01" @default.
- W3189656476 modified "2023-09-25" @default.
- W3189656476 title "Efficient and Rapid Analysis of Polysomes and Ribosomal Subunits in Cells and Tissues Using Ribo Mega-SEC" @default.
- W3189656476 cites W1494360400 @default.
- W3189656476 cites W1534237047 @default.
- W3189656476 cites W1975224651 @default.
- W3189656476 cites W2000310005 @default.
- W3189656476 cites W2037199116 @default.
- W3189656476 cites W2081090039 @default.
- W3189656476 cites W2095032938 @default.
- W3189656476 cites W2101990520 @default.
- W3189656476 cites W2124348590 @default.
- W3189656476 cites W2125695045 @default.
- W3189656476 cites W2160215304 @default.
- W3189656476 cites W2226665541 @default.
- W3189656476 cites W2586333749 @default.
- W3189656476 cites W2621126205 @default.
- W3189656476 cites W2625763766 @default.
- W3189656476 cites W2887745160 @default.
- W3189656476 cites W2891583395 @default.
- W3189656476 cites W2924359975 @default.
- W3189656476 cites W2950337279 @default.
- W3189656476 cites W2950420995 @default.
- W3189656476 cites W3159226327 @default.
- W3189656476 cites W3170458923 @default.
- W3189656476 cites W4210538738 @default.
- W3189656476 cites W4243250311 @default.
- W3189656476 doi "https://doi.org/10.21769/bioprotoc.4106" @default.
- W3189656476 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8376616" @default.
- W3189656476 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34458400" @default.
- W3189656476 hasPublicationYear "2021" @default.
- W3189656476 type Work @default.
- W3189656476 sameAs 3189656476 @default.
- W3189656476 citedByCount "2" @default.
- W3189656476 countsByYear W31896564762022 @default.
- W3189656476 countsByYear W31896564762023 @default.
- W3189656476 crossrefType "journal-article" @default.
- W3189656476 hasAuthorship W3189656476A5014112981 @default.
- W3189656476 hasAuthorship W3189656476A5019806968 @default.
- W3189656476 hasAuthorship W3189656476A5025927634 @default.
- W3189656476 hasAuthorship W3189656476A5032278821 @default.
- W3189656476 hasAuthorship W3189656476A5076477268 @default.
- W3189656476 hasBestOaLocation W31896564762 @default.
- W3189656476 hasConcept C104317684 @default.
- W3189656476 hasConcept C112755997 @default.
- W3189656476 hasConcept C185592680 @default.
- W3189656476 hasConcept C38062823 @default.
- W3189656476 hasConcept C43617362 @default.
- W3189656476 hasConcept C55493867 @default.
- W3189656476 hasConcept C65666806 @default.
- W3189656476 hasConcept C67705224 @default.
- W3189656476 hasConcept C67905577 @default.
- W3189656476 hasConcept C86803240 @default.
- W3189656476 hasConcept C88478588 @default.
- W3189656476 hasConcept C9893847 @default.
- W3189656476 hasConceptScore W3189656476C104317684 @default.
- W3189656476 hasConceptScore W3189656476C112755997 @default.
- W3189656476 hasConceptScore W3189656476C185592680 @default.
- W3189656476 hasConceptScore W3189656476C38062823 @default.
- W3189656476 hasConceptScore W3189656476C43617362 @default.
- W3189656476 hasConceptScore W3189656476C55493867 @default.
- W3189656476 hasConceptScore W3189656476C65666806 @default.
- W3189656476 hasConceptScore W3189656476C67705224 @default.
- W3189656476 hasConceptScore W3189656476C67905577 @default.
- W3189656476 hasConceptScore W3189656476C86803240 @default.
- W3189656476 hasConceptScore W3189656476C88478588 @default.
- W3189656476 hasConceptScore W3189656476C9893847 @default.
- W3189656476 hasIssue "15" @default.
- W3189656476 hasLocation W31896564761 @default.
- W3189656476 hasLocation W31896564762 @default.
- W3189656476 hasLocation W31896564763 @default.
- W3189656476 hasLocation W31896564764 @default.
- W3189656476 hasLocation W31896564765 @default.
- W3189656476 hasOpenAccess W3189656476 @default.
- W3189656476 hasPrimaryLocation W31896564761 @default.
- W3189656476 hasRelatedWork W1574378429 @default.
- W3189656476 hasRelatedWork W1912800334 @default.
- W3189656476 hasRelatedWork W1975107163 @default.
- W3189656476 hasRelatedWork W1996496481 @default.
- W3189656476 hasRelatedWork W2003562397 @default.
- W3189656476 hasRelatedWork W2034653428 @default.
- W3189656476 hasRelatedWork W2043566555 @default.
- W3189656476 hasRelatedWork W2097985443 @default.
- W3189656476 hasRelatedWork W2342486735 @default.
- W3189656476 hasRelatedWork W2183910007 @default.
- W3189656476 hasVolume "11" @default.
- W3189656476 isParatext "false" @default.
- W3189656476 isRetracted "false" @default.
- W3189656476 magId "3189656476" @default.
- W3189656476 workType "article" @default.