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• W3189846872 abstract "In this chapter, we describe the proteomic approach named Native Chromatin Proteomics (N-ChroP) that couples a modified Chromatin ImmunoPrecipitation (ChIP) protocol with the mass spectrometry (MS) analysis of immunoprecipitated proteins to study the combinatorial enrichment or exclusion of histone post-translational modifications (PTMs) at specific genomic regions, such as promoters or enhancers. We describe the protocol steps from the digestion of chromatin and nucleosome immunoprecipitation to histone digestion and peptide enrichment prior to MS analysis, up to the MS raw data analysis. We also discuss current challenges and offer suggestions based on the direct hands-on experience acquired during the method setup." @default.
• W3189846872 created "2021-08-16" @default.
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• W3189846872 date "2021-01-01" @default.
• W3189846872 modified "2023-10-12" @default.
• W3189846872 title "Native Chromatin Proteomics (N-ChroP) to Characterize Histone Post-translational Modification (PTM) Combinatorics at Distinct Genomic Regions" @default.
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• W3189846872 doi "https://doi.org/10.1007/978-1-0716-1597-3_14" @default.
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