Matches in SemOpenAlex for { <https://semopenalex.org/work/W3190147176> ?p ?o ?g. }
Showing items 1 to 66 of
66
with 100 items per page.
- W3190147176 abstract "Swift recognition ofdisease-causing pathogens at the point-of-care enables life-saving treatmentand infection control. However, current rapid diagnostic devices often fail todetect the low concentrations of pathogens present in the early stages ofinfection, causing delayed and even incorrect treatments. Rapid diagnosticsthat require multiple steps and/or elevated temperatures to perform have anumber of barriers to use at the point-of-care and in the field, and despiteefforts to simplify these platforms for ease of use, many still requirediagnostic-specific training for the healthcare professionals who use them.Most nucleic acid amplification assays require hours to perform in a sterilelaboratory setting that may be still more hours from a patient’s bedside or notat all feasible for transport in remote or low-resourced areas. The cold-chainstorage of reagents, multistep sample preparation, and costly instrumentationrequired to analyze samples has prohibited many nucleic acid detection andantibody-based assays from reaching the point-of-care. There remains a criticalneed to bring rapid and accessible pathogen identification technologies thatdetermine disease status and ensure effective treatment out of the laboratory.Paper-based diagnostics have emerged as a portable platform for antigenand nucleic acid detection of pathogens but are often limited by theirimperfect control of reagent incubation, multiple complex steps, andinconsistent false positive results. Here, I have developed mechanisms toeconomically improve thermal incubations, automate dried reagent flow formultistep assays, and specifically detect pathogenic antigens while improvingfinal output sensitivity on paper-based devices. First, I characterizeminiaturized inkjet printed joule-heaters (microheaters) that enable thermalcontrol for pathogen lysis and nucleic acid amplification incubation on alow-cost paper-based device. Next, I explore 2-Dimensional Paper Networks as ameans to automate multistep visual enhancement reactions with dried reagents toincrease the sensitivity and readability of nucleic acid detection withpaper-based devices. Lastly, I aim to create a novel Reverse-TranscriptionRecombinase Polymerase Reaction mechanism to amplify and detect a specificregion of the Spike protein domain of SARS-CoV-2. This will allow the rapiddetection of SARS-CoV-2 infections to aid in managing the current COVID-19 pandemic.In the future, these tools could be integrated into a rapid diagnostic test forSARS-CoV-2 and other pathogens, ultimately improving the accessibility andsensitivity of rapid diagnostics on multiple fronts." @default.
- W3190147176 created "2021-08-16" @default.
- W3190147176 creator A5032835961 @default.
- W3190147176 date "2021-07-28" @default.
- W3190147176 modified "2023-09-24" @default.
- W3190147176 title "Platforms and Molecular Mechanisms for Improving Signal Transduction and Signal Enhancement in Multi-step Point-Of-Care Diagnostics" @default.
- W3190147176 doi "https://doi.org/10.25394/pgs.15062847.v1" @default.
- W3190147176 hasPublicationYear "2021" @default.
- W3190147176 type Work @default.
- W3190147176 sameAs 3190147176 @default.
- W3190147176 citedByCount "0" @default.
- W3190147176 crossrefType "dissertation" @default.
- W3190147176 hasAuthorship W3190147176A5032835961 @default.
- W3190147176 hasConcept C116834253 @default.
- W3190147176 hasConcept C142724271 @default.
- W3190147176 hasConcept C203014093 @default.
- W3190147176 hasConcept C24107716 @default.
- W3190147176 hasConcept C2910367586 @default.
- W3190147176 hasConcept C41008148 @default.
- W3190147176 hasConcept C51915194 @default.
- W3190147176 hasConcept C55493867 @default.
- W3190147176 hasConcept C59822182 @default.
- W3190147176 hasConcept C64841741 @default.
- W3190147176 hasConcept C70721500 @default.
- W3190147176 hasConcept C71924100 @default.
- W3190147176 hasConcept C86803240 @default.
- W3190147176 hasConceptScore W3190147176C116834253 @default.
- W3190147176 hasConceptScore W3190147176C142724271 @default.
- W3190147176 hasConceptScore W3190147176C203014093 @default.
- W3190147176 hasConceptScore W3190147176C24107716 @default.
- W3190147176 hasConceptScore W3190147176C2910367586 @default.
- W3190147176 hasConceptScore W3190147176C41008148 @default.
- W3190147176 hasConceptScore W3190147176C51915194 @default.
- W3190147176 hasConceptScore W3190147176C55493867 @default.
- W3190147176 hasConceptScore W3190147176C59822182 @default.
- W3190147176 hasConceptScore W3190147176C64841741 @default.
- W3190147176 hasConceptScore W3190147176C70721500 @default.
- W3190147176 hasConceptScore W3190147176C71924100 @default.
- W3190147176 hasConceptScore W3190147176C86803240 @default.
- W3190147176 hasLocation W31901471761 @default.
- W3190147176 hasOpenAccess W3190147176 @default.
- W3190147176 hasPrimaryLocation W31901471761 @default.
- W3190147176 hasRelatedWork W121757546 @default.
- W3190147176 hasRelatedWork W1498307867 @default.
- W3190147176 hasRelatedWork W1966345333 @default.
- W3190147176 hasRelatedWork W1982179033 @default.
- W3190147176 hasRelatedWork W2128612221 @default.
- W3190147176 hasRelatedWork W2492191406 @default.
- W3190147176 hasRelatedWork W2547863259 @default.
- W3190147176 hasRelatedWork W2548046270 @default.
- W3190147176 hasRelatedWork W2620682849 @default.
- W3190147176 hasRelatedWork W2755987600 @default.
- W3190147176 hasRelatedWork W2783542638 @default.
- W3190147176 hasRelatedWork W2920363504 @default.
- W3190147176 hasRelatedWork W3011521271 @default.
- W3190147176 hasRelatedWork W3091127702 @default.
- W3190147176 hasRelatedWork W3113260903 @default.
- W3190147176 hasRelatedWork W3154674552 @default.
- W3190147176 hasRelatedWork W3194183867 @default.
- W3190147176 hasRelatedWork W3210796395 @default.
- W3190147176 hasRelatedWork W3212257653 @default.
- W3190147176 hasRelatedWork W3214606109 @default.
- W3190147176 isParatext "false" @default.
- W3190147176 isRetracted "false" @default.
- W3190147176 magId "3190147176" @default.
- W3190147176 workType "dissertation" @default.