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• W3190404824 endingPage "130586" @default.
• W3190404824 startingPage "130586" @default.
• W3190404824 abstract "• A smartphone-read G-quadruplex-based CRISPR-Cas12a bioassay for ultrasensitive and selective detection of bacteria has been developed. • The limit of detection (LOD) of Salmonella was 1 CFU/mL with a dynamic detection range from 10 0 to 10 8 CFU/mL. • Our proposed bioassay represented high selectivity for Salmonella against other interfering cells. • It balanced rapidity, sensitivity, specificity, POCT capability and was successfully applied for detecting Salmonella in food samples. Foodborne diseases, caused by pathogenic bacteria, severely threaten global human health and cause a financial burden. Rapid, sensitive and on-site detection of pathogenic bacteria is significant. The existing methods have different defects, such as time-consuming and inconvenient. In this study, we developed a G-quadruplex-based CRISPR-Cas12a bioassay for pathogenic bacteria detection with high sensitivity and visualization capability. Salmonella was used as the detection model. Simply, the amplicons of Salmonella specific invA gene activated the trans -cleavage activity of Cas12a and triggered CRISPR-Cas12a based indiscriminate degradation of single-stranded DNAs (ssDNAs). The ssDNAs were designed with the guanine-rich sequence and formed a stable G-quadruplex DNAzyme by adding K + . This DNAzyme could catalyze the TMB-H 2 O 2 reaction in the presence of hemin, leading to an increase in absorbance at 454 nm and a color change. This change can be readily differentiated by the naked eyes as well as a smartphone with a Color Picker App. With this strategy, the limit of detection (LOD) for Salmonella was 1 CFU/mL with no cross-reactivity. A linear relationship (R 2 = 0.993) between the absorbance and the concentration of Salmonella was obtained. Furthermore, G-quadruplex-based CRISPR-Cas12a bioassay was successfully applied for sensing Salmonella in real food samples. This work not only expands the reach of CRISPR-Cas based biosensing but also provides a novel pathogenic bacteria detection method with high sensitivity, specificity and on-site capability." @default.
• W3190404824 created "2021-08-16" @default.
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• W3190404824 date "2021-11-01" @default.
• W3190404824 modified "2023-10-16" @default.
• W3190404824 title "Ultrasensitive pathogenic bacteria detection by a smartphone-read G-quadruplex-based CRISPR-Cas12a bioassay" @default.
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• W3190404824 doi "https://doi.org/10.1016/j.snb.2021.130586" @default.
• W3190404824 hasPublicationYear "2021" @default.
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