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- W3190480788 abstract "The purpose of this study was to improve the frozen-thawed sperm of Korean Native Cattle using magnetized water. The semen was collected by artificial vagina. Before cryopreservation, Triladyl was flowed through magnet [0, 2000, 4000 and 6000 Gauss (G)] for 5min. The semen was diluted to magnetized Triladyl with 20% egg-yolk for freezing. The diluted semen was placed in 0.5ml straws, and freezing process was exposed on ‒120℃ for 10min. Diluted sperm was stored into LN2. Analysis of frozen- thawed sperm was estimated of viability with SYBR14/PI stain, and membrane intact with hypoosmotic swelling test (HOST). The mitochondrial function analysis was conducted by staining with Rhodamin 123 by flow cytometry and was analyzed histogram. The intensity of acrosome damage was analyzed using FITC-PNA stain by flow cytometry. Analysis of rhodamin 123 and FITC-PNA stain were used mitochondria and acrosome membrane intact. As a results, sperm viability was significantly higher in 4000G (76.0±1.2%) group than other groups (p<0.05). However, HOST was significantly higher in 4000 (37.7±0.6%) and 6000G (35.0±1.1%) than 0 (30.3±0.9%) and 2000G (30.7±0.5%) (p<0.05). In addition, mitochondria membrane and acrosome damage were significantly lower in 6000G (1.40±0.08% and 26.7±2.9%) group than other groups (p<0.05). In conclusion we suggest that magnetized water could improve the ability of sperm on cryopreservation of korean native cattle. * This work was supported by Grant No. PJ 907008 from Rural development administration (RDA)." @default.
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- W3190480788 date "2011-10-01" @default.
- W3190480788 modified "2023-09-23" @default.
- W3190480788 title "Effect of Magnetized Water on Cryopreservation in Bovine Spermatozoa" @default.
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