Matches in SemOpenAlex for { <https://semopenalex.org/work/W3190646979> ?p ?o ?g. }
Showing items 1 to 67 of
67
with 100 items per page.
- W3190646979 abstract "As tuberculosis is still the number one leading cause of death in humans due to a single infectious agent, prevention of infection is a key tactic in fighting it.Even though the vaccine BCG exists for tuberculosis since the beginning of the 20thcen-tury, it lacks efficacy and does not provide sustainable protection.An important step to develop a long-term immunity is the expansion of antigen-specific T cells in the lymph node. For T-cell priming to occur, the pathogen and/or its antigen has to be transported from the site of infection to the T-cell zone of the draining lymph node. This step is done by migratory Dendritic cells (DCs). DCs reside as sentinels in tis-sue, where after pathogen encounter, internalize the pathogen, process its antigens and migrate to the draining lymph node.Using a modified protocol, developed by Bollampali et al. (2015), during a previous study done Dr. Rothfuchs team, we investigated the mechanisms involved in the migration of DCs after injection of BCG. For this we injected mice with different inhibitors and recep-tor antagonists. It is worth mentioning that in a yet unpublished previous work we in-hibited the enzymes COX-1 and COX-2 systemically, using indomethacin, and showed a decreased number of DCs migrating to the draining popliteal lymph node after BCG in-jection compared to mice not injected with the inhibitor.Following our experiment, we decided to investigate the receptors of one of the down-stream products of COX, PGE2 and the corresponding receptors EP2 and EP4. Based on other studies two of the four receptors of PGE2, EP2 and EP4, are important for the mi-gration of DCs. We then injected mice with antagonists for these two either separate or simultaneously. Even though their affinities and the sustainability of the reaction caus-ing PGE2signalling are different, both receptors were able to compensate for each other, but a decreased number of DCs were found in the lymph node when both antagonists were injected. This and the results of the COX inhibition suggest a role for PGE2 on DC migration.Based on these results we decided to look at MMP-9, a molecule regulated by PGE2 sig-nalling. However, inhibition of MMP-9 in our model did not impact on DC migration. It is unclear if treatment with the MMP-9 inhibitor used failed or if MMP-9 is indeed not important in BCG-triggered DC migration.In conclusion in this thesis we show a role for EP2 and EP4 on DC migration after BCG injection but failed to show the same for MMP-9." @default.
- W3190646979 created "2021-08-16" @default.
- W3190646979 creator A5072220318 @default.
- W3190646979 date "2020-01-01" @default.
- W3190646979 modified "2023-09-24" @default.
- W3190646979 title "Regulation of dendritic migration in response to BCG" @default.
- W3190646979 hasPublicationYear "2020" @default.
- W3190646979 type Work @default.
- W3190646979 sameAs 3190646979 @default.
- W3190646979 citedByCount "0" @default.
- W3190646979 crossrefType "journal-article" @default.
- W3190646979 hasAuthorship W3190646979A5072220318 @default.
- W3190646979 hasConcept C100701293 @default.
- W3190646979 hasConcept C142724271 @default.
- W3190646979 hasConcept C147483822 @default.
- W3190646979 hasConcept C203014093 @default.
- W3190646979 hasConcept C2777863537 @default.
- W3190646979 hasConcept C2778170410 @default.
- W3190646979 hasConcept C2779720271 @default.
- W3190646979 hasConcept C2780849966 @default.
- W3190646979 hasConcept C2781069245 @default.
- W3190646979 hasConcept C59822182 @default.
- W3190646979 hasConcept C71924100 @default.
- W3190646979 hasConcept C81444415 @default.
- W3190646979 hasConcept C86803240 @default.
- W3190646979 hasConcept C8891405 @default.
- W3190646979 hasConceptScore W3190646979C100701293 @default.
- W3190646979 hasConceptScore W3190646979C142724271 @default.
- W3190646979 hasConceptScore W3190646979C147483822 @default.
- W3190646979 hasConceptScore W3190646979C203014093 @default.
- W3190646979 hasConceptScore W3190646979C2777863537 @default.
- W3190646979 hasConceptScore W3190646979C2778170410 @default.
- W3190646979 hasConceptScore W3190646979C2779720271 @default.
- W3190646979 hasConceptScore W3190646979C2780849966 @default.
- W3190646979 hasConceptScore W3190646979C2781069245 @default.
- W3190646979 hasConceptScore W3190646979C59822182 @default.
- W3190646979 hasConceptScore W3190646979C71924100 @default.
- W3190646979 hasConceptScore W3190646979C81444415 @default.
- W3190646979 hasConceptScore W3190646979C86803240 @default.
- W3190646979 hasConceptScore W3190646979C8891405 @default.
- W3190646979 hasLocation W31906469791 @default.
- W3190646979 hasOpenAccess W3190646979 @default.
- W3190646979 hasPrimaryLocation W31906469791 @default.
- W3190646979 hasRelatedWork W134230035 @default.
- W3190646979 hasRelatedWork W1486222795 @default.
- W3190646979 hasRelatedWork W1543727885 @default.
- W3190646979 hasRelatedWork W1560282990 @default.
- W3190646979 hasRelatedWork W168020489 @default.
- W3190646979 hasRelatedWork W1972238102 @default.
- W3190646979 hasRelatedWork W1988558327 @default.
- W3190646979 hasRelatedWork W1988843622 @default.
- W3190646979 hasRelatedWork W2039155569 @default.
- W3190646979 hasRelatedWork W2042863325 @default.
- W3190646979 hasRelatedWork W2125760866 @default.
- W3190646979 hasRelatedWork W2130166359 @default.
- W3190646979 hasRelatedWork W2139133924 @default.
- W3190646979 hasRelatedWork W2255098585 @default.
- W3190646979 hasRelatedWork W2335949741 @default.
- W3190646979 hasRelatedWork W2592953665 @default.
- W3190646979 hasRelatedWork W2766732330 @default.
- W3190646979 hasRelatedWork W2795853829 @default.
- W3190646979 hasRelatedWork W2901804749 @default.
- W3190646979 hasRelatedWork W3002326815 @default.
- W3190646979 isParatext "false" @default.
- W3190646979 isRetracted "false" @default.
- W3190646979 magId "3190646979" @default.
- W3190646979 workType "article" @default.