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- W3190997651 abstract "Single-nucleus RNA sequencing allows the profiling of gene expression in isolated nuclei. Here, we describe a step-by-step protocol optimized for adult mouse skeletal muscles. This protocol provides two main advantages compared to the widely used single-cell protocol. First, it allows us to sequence the myonuclei of the multinucleated myofibers. Second, it circumvents the cell-dissociation-induced transcriptional modifications. For complete details on the use and execution of this protocol, please refer to Dos Santos et al. (2020) and Machado, Geara et al. (2021)." @default.
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- W3190997651 date "2021-09-01" @default.
- W3190997651 modified "2023-10-17" @default.
- W3190997651 title "Extraction and sequencing of single nuclei from murine skeletal muscles" @default.
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- W3190997651 doi "https://doi.org/10.1016/j.xpro.2021.100694" @default.
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