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- W3191039831 abstract "The model of tertiary of β-conglycinin beta-subunit was constructed by the bioinformation tool SWISS-MODEL, and the conformational epitopes were predicted by Disco Tope 2.0 server.The overlapping fragments of β-conglycinin beta-subunit genes were amplified by PCR and inserted into p MD-18 T vector, then preserved into competent cell of Escherichia coli JM109.The recombinant plasmids were evaluated by blue-white selection, PCR and double enzyme digestion, and DNA sequence analysis showed that the cloned sequence was the very fragment designed. The successful cloning of the genes can provide a reference for the study of the expresstion of β-conglycinin beta-subunit and screening of antigenic epitopes." @default.
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- W3191039831 date "2017-01-01" @default.
- W3191039831 modified "2023-09-24" @default.
- W3191039831 title "Analysis of antigenic epitopes and cloning of soybean major allergen β-conglycinin beta-subunit" @default.
- W3191039831 doi "https://doi.org/10.13386/j.issn1002-0306.2017.18.018" @default.
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