FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3191136083> ?p ?o ?g. }

• W3191136083 abstract "The aging of the population has led to an annual increase in the incidence of vascular calcification (VC). Specific protein 1 (Sp1) is a transcriptional activator that serves an important role in VC. The deacetylation of transcription factors represses their binding to the promoters of downstream genes, thereby causing their downregulation. The present study aimed to investigate the role of deacetylated Sp1 in the development of VC. In the present study, western blotting and immunoprecipitation (IP) were performed to detect the protein levels of acetylated Sp1. Western blotting and immunofluorescence staining were used to analyze phenotypic switching in vascular smooth muscle cells (VSMCs). Alizarin red S, alkaline phosphatase (ALP) activity and calcium content assays were used to assess calcium deposition in VSMCs. Western blotting, flow cytometry, TUNEL staining and caspase3 activity assay were used to evaluate apoptosis of VSMCs. Chromatin immunoprecipitation (ChIP) assay was used to detect Sp1 binding to the BMP2 promoter. The results indicated that, in a β‑glycerophosphate (β‑GP)‑induced VSMC calcification model, the level of acetylated Sp1 was increased. Western blotting and immunofluorescence staining results showed that, compared with the Sp1 overexpression group (Sp1‑WT), deacetylated Sp1 (Sp1‑K704A) downregulated the expression of osteogenic markers runt‑related transcription factor 2 (Runx2) and bone morphogenetic protein 2 (BMP2), and upregulated the expression of contraction marker α‑smooth muscle actin (α‑SMA) and calponin 1. In addition, deacetylated Sp1 also reduced the ALP activity and calcium content of calcified VSMCs, and the Alizarin red S assay revealed that the calcium crystallization of Sp1‑K704A group was markedly decreased. Western blotting, flow cytometry, TUNEL staining and caspase‑3 activity assay were detected to indicate that the B‑cell lymphoma 2 (Bcl‑2)/Bcl‑2‑associated X protein ratio was increased, and caspase‑3 activity and the apoptotic rate of VSMCs were decreased, in the Sp1‑K704A group, as compared with the Sp1‑WT group. ChIP assay revealed that Sp1 binding to the BMP2 promoter was downregulated in the Sp1‑K704A group, compared with that in theSp1‑WT group. In conclusion, a deacetylated mutant of Sp1 decreased Sp1 binding to the BMP2 promoter, thus decreasing apoptosis, phenotypic switching and calcium deposition in calcified VSMCs. This finding may indicate potential therapeutic targets for VC." @default.
• W3191136083 created "2021-08-16" @default.
• W3191136083 creator A5010776860 @default.
• W3191136083 creator A5022381559 @default.
• W3191136083 creator A5028325404 @default.
• W3191136083 creator A5046173951 @default.
• W3191136083 creator A5049783149 @default.
• W3191136083 creator A5053815236 @default.
• W3191136083 creator A5060244431 @default.
• W3191136083 creator A5067341731 @default.
• W3191136083 creator A5073216396 @default.
• W3191136083 creator A5080378808 @default.
• W3191136083 date "2021-08-10" @default.
• W3191136083 modified "2023-09-28" @default.
• W3191136083 title "Deacetylated Sp1 improves β‑glycerophosphate‑induced calcification in vascular smooth muscle cells" @default.
• W3191136083 cites W1970632084 @default.
• W3191136083 cites W1978209251 @default.
• W3191136083 cites W1979055405 @default.
• W3191136083 cites W1989382448 @default.
• W3191136083 cites W2019732936 @default.
• W3191136083 cites W2036370282 @default.
• W3191136083 cites W2061046080 @default.
• W3191136083 cites W2063124267 @default.
• W3191136083 cites W2099356747 @default.
• W3191136083 cites W2108116509 @default.
• W3191136083 cites W2133778193 @default.
• W3191136083 cites W2150762378 @default.
• W3191136083 cites W2159479890 @default.
• W3191136083 cites W2162075091 @default.
• W3191136083 cites W2163691924 @default.
• W3191136083 cites W2201671538 @default.
• W3191136083 cites W2591421310 @default.
• W3191136083 cites W2790654167 @default.
• W3191136083 cites W2793107094 @default.
• W3191136083 cites W2829665001 @default.
• W3191136083 cites W2910244702 @default.
• W3191136083 cites W2922795099 @default.
• W3191136083 cites W2943311733 @default.
• W3191136083 cites W2947149863 @default.
• W3191136083 cites W2969381862 @default.
• W3191136083 cites W2990424442 @default.
• W3191136083 cites W3005037272 @default.
• W3191136083 cites W3021544222 @default.
• W3191136083 cites W3025523251 @default.
• W3191136083 cites W3034816748 @default.
• W3191136083 cites W3045569242 @default.
• W3191136083 cites W3047593357 @default.
• W3191136083 cites W3083828841 @default.
• W3191136083 cites W4233947395 @default.
• W3191136083 doi "https://doi.org/10.3892/etm.2021.10586" @default.
• W3191136083 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8394101" @default.
• W3191136083 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34504597" @default.
• W3191136083 hasPublicationYear "2021" @default.
• W3191136083 type Work @default.
• W3191136083 sameAs 3191136083 @default.
• W3191136083 citedByCount "2" @default.
• W3191136083 countsByYear W31911360832022 @default.
• W3191136083 crossrefType "journal-article" @default.
• W3191136083 hasAuthorship W3191136083A5010776860 @default.
• W3191136083 hasAuthorship W3191136083A5022381559 @default.
• W3191136083 hasAuthorship W3191136083A5028325404 @default.
• W3191136083 hasAuthorship W3191136083A5046173951 @default.
• W3191136083 hasAuthorship W3191136083A5049783149 @default.
• W3191136083 hasAuthorship W3191136083A5053815236 @default.
• W3191136083 hasAuthorship W3191136083A5060244431 @default.
• W3191136083 hasAuthorship W3191136083A5067341731 @default.
• W3191136083 hasAuthorship W3191136083A5073216396 @default.
• W3191136083 hasAuthorship W3191136083A5080378808 @default.
• W3191136083 hasBestOaLocation W31911360831 @default.
• W3191136083 hasConcept C101762097 @default.
• W3191136083 hasConcept C104317684 @default.
• W3191136083 hasConcept C127561419 @default.
• W3191136083 hasConcept C134018914 @default.
• W3191136083 hasConcept C134320426 @default.
• W3191136083 hasConcept C150194340 @default.
• W3191136083 hasConcept C153911025 @default.
• W3191136083 hasConcept C160160445 @default.
• W3191136083 hasConcept C181199279 @default.
• W3191136083 hasConcept C2776363554 @default.
• W3191136083 hasConcept C2779395532 @default.
• W3191136083 hasConcept C2780804394 @default.
• W3191136083 hasConcept C2992686903 @default.
• W3191136083 hasConcept C55493867 @default.
• W3191136083 hasConcept C85265743 @default.
• W3191136083 hasConcept C86803240 @default.
• W3191136083 hasConceptScore W3191136083C101762097 @default.
• W3191136083 hasConceptScore W3191136083C104317684 @default.
• W3191136083 hasConceptScore W3191136083C127561419 @default.
• W3191136083 hasConceptScore W3191136083C134018914 @default.
• W3191136083 hasConceptScore W3191136083C134320426 @default.
• W3191136083 hasConceptScore W3191136083C150194340 @default.
• W3191136083 hasConceptScore W3191136083C153911025 @default.
• W3191136083 hasConceptScore W3191136083C160160445 @default.
• W3191136083 hasConceptScore W3191136083C181199279 @default.
• W3191136083 hasConceptScore W3191136083C2776363554 @default.
• W3191136083 hasConceptScore W3191136083C2779395532 @default.
• W3191136083 hasConceptScore W3191136083C2780804394 @default.
• W3191136083 hasConceptScore W3191136083C2992686903 @default.
• W3191136083 hasConceptScore W3191136083C55493867 @default.
• W3191136083 hasConceptScore W3191136083C85265743 @default.

• next