FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3191277162> ?p ?o ?g. }

• W3191277162 abstract "The endothelial cells which form the inner cellular lining of the vasculature can act as non-professional phagocytes to ingest and remove emboli and aged/injured red blood cells (RBCs) from circulation. We previously demonstrated an erythrophagocytic phenotype of the brain endothelium for oxidatively stressed RBCs with subsequent migration of iron-rich RBCs and RBC degradation products across the brain endothelium in vivo and in vitro , in the absence of brain endothelium disruption. However, the mechanisms contributing to brain endothelial erythrophagocytosis are not well defined, and herein we elucidate the cellular mechanisms underlying brain endothelial erythrophagocytosis. Murine brain microvascular endothelial cells (bEnd.3 cells) were incubated with tert-butyl hydroperoxide (tBHP, oxidative stressor to induce RBC aging in vitro )- or PBS (control)-treated mouse RBCs. tBHP increased the reactive oxygen species (ROS) formation and phosphatidylserine exposure in RBCs, which were associated with robust brain endothelial erythrophagocytosis. TNFα treatment potentiated the brain endothelial erythrophagocytosis of tBHP-RBCs in vitro . Brain endothelial erythrophagocytosis was significantly reduced by RBC phosphatidylserine cloaking with annexin-V and with RBC-ROS and phosphatidylserine reduction with vitamin C. Brain endothelial erythrophagocytosis did not alter the bEnd.3 viability, and tBHP-RBCs were localized with early and late endosomes. Brain endothelial erythrophagocytosis increased the bEnd.3 total iron pool, abluminal iron levels without causing brain endothelial monolayer disruption, and ferroportin levels. In vivo , intravenous tBHP-RBC injection in aged (17–18 months old) male C57BL/6 mice significantly increased the Prussian blue-positive iron-rich lesion load compared with PBS-RBC-injected mice. In conclusion, RBC phosphatidylserine exposure and ROS are key mediators of brain endothelial erythrophagocytosis, a process which is associated with increased abluminal iron in vitro . tBHP-RBCs result in Prussian blue-positive iron-rich lesions in vivo . Brain endothelial erythrophagocytosis may provide a new route for RBC/RBC degradation product entry into the brain to produce iron-rich cerebral microhemorrhage-like lesions." @default.
• W3191277162 created "2021-08-16" @default.
• W3191277162 creator A5011794759 @default.
• W3191277162 creator A5027852438 @default.
• W3191277162 creator A5028391342 @default.
• W3191277162 creator A5030274546 @default.
• W3191277162 creator A5065067002 @default.
• W3191277162 creator A5066252030 @default.
• W3191277162 creator A5076871855 @default.
• W3191277162 creator A5083245275 @default.
• W3191277162 creator A5090252275 @default.
• W3191277162 date "2021-08-02" @default.
• W3191277162 modified "2023-09-25" @default.
• W3191277162 title "Insights Into the Mechanisms of Brain Endothelial Erythrophagocytosis" @default.
• W3191277162 cites W1493342528 @default.
• W3191277162 cites W1532446175 @default.
• W3191277162 cites W1795220919 @default.
• W3191277162 cites W1964718511 @default.
• W3191277162 cites W1964791394 @default.
• W3191277162 cites W1968412972 @default.
• W3191277162 cites W1978373692 @default.
• W3191277162 cites W1979621257 @default.
• W3191277162 cites W1987113388 @default.
• W3191277162 cites W1999987471 @default.
• W3191277162 cites W2002847421 @default.
• W3191277162 cites W2014321361 @default.
• W3191277162 cites W2025559349 @default.
• W3191277162 cites W2030529426 @default.
• W3191277162 cites W2052569658 @default.
• W3191277162 cites W2072735798 @default.
• W3191277162 cites W2076846521 @default.
• W3191277162 cites W2088080018 @default.
• W3191277162 cites W2094724889 @default.
• W3191277162 cites W2103818266 @default.
• W3191277162 cites W2104704429 @default.
• W3191277162 cites W2117854054 @default.
• W3191277162 cites W2124609728 @default.
• W3191277162 cites W2145109289 @default.
• W3191277162 cites W2147743457 @default.
• W3191277162 cites W2148568506 @default.
• W3191277162 cites W2158387087 @default.
• W3191277162 cites W2163770183 @default.
• W3191277162 cites W2179649617 @default.
• W3191277162 cites W2515130689 @default.
• W3191277162 cites W2584561567 @default.
• W3191277162 cites W2772514947 @default.
• W3191277162 cites W2774662704 @default.
• W3191277162 cites W2790215203 @default.
• W3191277162 cites W2808384893 @default.
• W3191277162 cites W2809601308 @default.
• W3191277162 cites W2891876754 @default.
• W3191277162 cites W2944741800 @default.
• W3191277162 cites W2982177131 @default.
• W3191277162 cites W3041311129 @default.
• W3191277162 cites W3087037511 @default.
• W3191277162 cites W3103676929 @default.
• W3191277162 cites W3123802905 @default.
• W3191277162 doi "https://doi.org/10.3389/fcell.2021.672009" @default.
• W3191277162 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8365766" @default.
• W3191277162 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34409026" @default.
• W3191277162 hasPublicationYear "2021" @default.
• W3191277162 type Work @default.
• W3191277162 sameAs 3191277162 @default.
• W3191277162 citedByCount "3" @default.
• W3191277162 countsByYear W31912771622022 @default.
• W3191277162 countsByYear W31912771622023 @default.
• W3191277162 crossrefType "journal-article" @default.
• W3191277162 hasAuthorship W3191277162A5011794759 @default.
• W3191277162 hasAuthorship W3191277162A5027852438 @default.
• W3191277162 hasAuthorship W3191277162A5028391342 @default.
• W3191277162 hasAuthorship W3191277162A5030274546 @default.
• W3191277162 hasAuthorship W3191277162A5065067002 @default.
• W3191277162 hasAuthorship W3191277162A5066252030 @default.
• W3191277162 hasAuthorship W3191277162A5076871855 @default.
• W3191277162 hasAuthorship W3191277162A5083245275 @default.
• W3191277162 hasAuthorship W3191277162A5090252275 @default.
• W3191277162 hasBestOaLocation W31912771621 @default.
• W3191277162 hasConcept C123012128 @default.
• W3191277162 hasConcept C134018914 @default.
• W3191277162 hasConcept C150903083 @default.
• W3191277162 hasConcept C169760540 @default.
• W3191277162 hasConcept C202751555 @default.
• W3191277162 hasConcept C203014093 @default.
• W3191277162 hasConcept C204232928 @default.
• W3191277162 hasConcept C207001950 @default.
• W3191277162 hasConcept C2776246508 @default.
• W3191277162 hasConcept C2776436680 @default.
• W3191277162 hasConcept C2776992346 @default.
• W3191277162 hasConcept C2778402981 @default.
• W3191277162 hasConcept C2778918659 @default.
• W3191277162 hasConcept C2779637612 @default.
• W3191277162 hasConcept C41625074 @default.
• W3191277162 hasConcept C529278444 @default.
• W3191277162 hasConcept C55493867 @default.
• W3191277162 hasConcept C86803240 @default.
• W3191277162 hasConcept C95444343 @default.
• W3191277162 hasConceptScore W3191277162C123012128 @default.
• W3191277162 hasConceptScore W3191277162C134018914 @default.
• W3191277162 hasConceptScore W3191277162C150903083 @default.
• W3191277162 hasConceptScore W3191277162C169760540 @default.

• next