Matches in SemOpenAlex for { <https://semopenalex.org/work/W3191418234> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W3191418234 endingPage "iii4" @default.
- W3191418234 startingPage "iii4" @default.
- W3191418234 abstract "Abstract The triple-negative breast cancer (TNBC) is the most malignant among breast cancers and has the high risk of developing metastasis into the brain. Metastases of breast cancers are increasing and pose a clinical challenge as the current treatments are not effective due to the unique brain microenvironment for metastatic breast cancer cells. While the contribution of brain macrophages to the formation of the metastatic niche is established, factors responsible for the crosstalk between cells remain elusive. SPP1 encoding a secreted phosphoprotein 1 (ostepontin) is highly overexpressed in malignant breast cancers. We evaluated the role of SPP1 in invasion and metastasis of human breast cancer cells. We found the increased invasion of triple-negative MDA-MB-231 (MDA-231) cells in the presence of human microglial HMSV40 cells. Using Western blot analysis demonstrated the elevated levels of focal adhesion kinase (FAK) and signal transducer and activator of transcription 3 (STAT3) in MDA-231 cells in co-cultures. Moreover, blocking SPP1 and integrin interactions with the synthetic RGD peptide, efficiently diminished both basic and microglia-induced invasion of MDA-231. To assess the role of SPP1 in cell invasion, we established the MDA-231 cells with knocked-down SPP1 expression using shRNA (shSPP1). Interestingly, the shSPP1 cells were unresponsive towards HMSV40 microglia. We have previously found that an antibiotic minocycline reduces SPP1 expression in glioma cells. We performed cell toxicity studies on 4 breast cancer cell lines and various non-malignant cells. All tested malignant cancer cells were more sensitize to minocycline than non-cancerous cells and breast cancer cells derived from TNBC were the most susceptible. Altogether, we demonstrate that microglia support invasion of breast cancer cells via SPP1/osteopontin triggering the integrin signalling, and minocycline by downregulating SPP1 expression may reduce both basic and microglia-induced cancer invasion. Therefore, we purpose that minocycline could be a new therapeutics targeting metastatic brain cancers." @default.
- W3191418234 created "2021-08-16" @default.
- W3191418234 creator A5020960668 @default.
- W3191418234 creator A5021591011 @default.
- W3191418234 creator A5024542401 @default.
- W3191418234 creator A5026045777 @default.
- W3191418234 creator A5039536580 @default.
- W3191418234 creator A5050446496 @default.
- W3191418234 creator A5052133430 @default.
- W3191418234 creator A5077156447 @default.
- W3191418234 date "2021-08-01" @default.
- W3191418234 modified "2023-09-30" @default.
- W3191418234 title "BSCI-15. Osteopontin plays a crucial role in invasiveness of triple negative breast cancer cells in the context of human microglia" @default.
- W3191418234 doi "https://doi.org/10.1093/noajnl/vdab071.014" @default.
- W3191418234 hasPublicationYear "2021" @default.
- W3191418234 type Work @default.
- W3191418234 sameAs 3191418234 @default.
- W3191418234 citedByCount "0" @default.
- W3191418234 crossrefType "journal-article" @default.
- W3191418234 hasAuthorship W3191418234A5020960668 @default.
- W3191418234 hasAuthorship W3191418234A5021591011 @default.
- W3191418234 hasAuthorship W3191418234A5024542401 @default.
- W3191418234 hasAuthorship W3191418234A5026045777 @default.
- W3191418234 hasAuthorship W3191418234A5039536580 @default.
- W3191418234 hasAuthorship W3191418234A5050446496 @default.
- W3191418234 hasAuthorship W3191418234A5052133430 @default.
- W3191418234 hasAuthorship W3191418234A5077156447 @default.
- W3191418234 hasBestOaLocation W31914182341 @default.
- W3191418234 hasConcept C121608353 @default.
- W3191418234 hasConcept C126322002 @default.
- W3191418234 hasConcept C142724271 @default.
- W3191418234 hasConcept C203014093 @default.
- W3191418234 hasConcept C2775930923 @default.
- W3191418234 hasConcept C2776914184 @default.
- W3191418234 hasConcept C2779013556 @default.
- W3191418234 hasConcept C2779830541 @default.
- W3191418234 hasConcept C2780110267 @default.
- W3191418234 hasConcept C502942594 @default.
- W3191418234 hasConcept C530470458 @default.
- W3191418234 hasConcept C71924100 @default.
- W3191418234 hasConcept C86803240 @default.
- W3191418234 hasConcept C96232424 @default.
- W3191418234 hasConceptScore W3191418234C121608353 @default.
- W3191418234 hasConceptScore W3191418234C126322002 @default.
- W3191418234 hasConceptScore W3191418234C142724271 @default.
- W3191418234 hasConceptScore W3191418234C203014093 @default.
- W3191418234 hasConceptScore W3191418234C2775930923 @default.
- W3191418234 hasConceptScore W3191418234C2776914184 @default.
- W3191418234 hasConceptScore W3191418234C2779013556 @default.
- W3191418234 hasConceptScore W3191418234C2779830541 @default.
- W3191418234 hasConceptScore W3191418234C2780110267 @default.
- W3191418234 hasConceptScore W3191418234C502942594 @default.
- W3191418234 hasConceptScore W3191418234C530470458 @default.
- W3191418234 hasConceptScore W3191418234C71924100 @default.
- W3191418234 hasConceptScore W3191418234C86803240 @default.
- W3191418234 hasConceptScore W3191418234C96232424 @default.
- W3191418234 hasIssue "Supplement_3" @default.
- W3191418234 hasLocation W31914182341 @default.
- W3191418234 hasOpenAccess W3191418234 @default.
- W3191418234 hasPrimaryLocation W31914182341 @default.
- W3191418234 hasRelatedWork W2154708791 @default.
- W3191418234 hasRelatedWork W2550381004 @default.
- W3191418234 hasRelatedWork W2898196535 @default.
- W3191418234 hasRelatedWork W2904294837 @default.
- W3191418234 hasRelatedWork W3033874287 @default.
- W3191418234 hasRelatedWork W3207768405 @default.
- W3191418234 hasRelatedWork W4282970457 @default.
- W3191418234 hasRelatedWork W4283771460 @default.
- W3191418234 hasRelatedWork W4304116770 @default.
- W3191418234 hasRelatedWork W4362546939 @default.
- W3191418234 hasVolume "3" @default.
- W3191418234 isParatext "false" @default.
- W3191418234 isRetracted "false" @default.
- W3191418234 magId "3191418234" @default.
- W3191418234 workType "article" @default.