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• W3192150044 abstract "HomePlant DiseaseVol. 106, No. 2First Report of Pectobacterium polaris Causing Aerial Stem Rot of Potato in China PreviousNext DISEASE NOTE OPENOpen Access licenseFirst Report of Pectobacterium polaris Causing Aerial Stem Rot of Potato in ChinaJinhui Wang, Wanxin Han, Zheng Li, Jianing Cheng, Yang Pan, Dongmei Zhao, Dai Zhang, Qian Li, Zhihui Yang, and Jiehua ZhuJinhui Wanghttps://orcid.org/0000-0002-4328-984XHebei Agricultural University, College of Plant Protection, Baoding 071001, ChinaSearch for more papers by this author, Wanxin Hanhttps://orcid.org/0000-0002-6037-8027Hebei Agricultural University, College of Plant Protection, Baoding 071001, ChinaSearch for more papers by this author, Zheng LiHebei Agricultural University, College of Plant Protection, Baoding 071001, ChinaSearch for more papers by this author, Jianing ChengHebei Agricultural University, Agricultural Business Training and Entrepreneurship Center, Baoding 071001, ChinaSearch for more papers by this author, Yang PanHebei Agricultural University, College of Plant Protection, Baoding 071001, ChinaSearch for more papers by this author, Dongmei Zhaohttps://orcid.org/0000-0002-8841-0914Hebei Agricultural University, College of Plant Protection, Baoding 071001, ChinaSearch for more papers by this author, Dai Zhanghttps://orcid.org/0000-0001-6871-0090Hebei Agricultural University, College of Plant Protection, Baoding 071001, ChinaSearch for more papers by this author, Qian LiHebei Agricultural University, College of Plant Protection, Baoding 071001, ChinaSearch for more papers by this author, Zhihui Yang†Corresponding authors: Z. Yang; E-mail Address: bdyzh@hebau.edu.cn, and J. Zhu; E-mail Address: zhujiehua@hebau.edu.cnHebei Agricultural University, College of Plant Protection, Baoding 071001, ChinaTechnological Innovation Center for Biological Control of Crop Diseases and Insect Pests of Hebei Province, Baoding 071001, ChinaSearch for more papers by this author, and Jiehua Zhu†Corresponding authors: Z. Yang; E-mail Address: bdyzh@hebau.edu.cn, and J. Zhu; E-mail Address: zhujiehua@hebau.edu.cnHebei Agricultural University, College of Plant Protection, Baoding 071001, ChinaTechnological Innovation Center for Biological Control of Crop Diseases and Insect Pests of Hebei Province, Baoding 071001, ChinaSearch for more papers by this author AffiliationsAuthors and Affiliations Jinhui Wang1 Wanxin Han1 Zheng Li1 Jianing Cheng2 Yang Pan1 Dongmei Zhao1 Dai Zhang1 Qian Li1 Zhihui Yang1 3 † Jiehua Zhu1 3 † 1Hebei Agricultural University, College of Plant Protection, Baoding 071001, China 2Hebei Agricultural University, Agricultural Business Training and Entrepreneurship Center, Baoding 071001, China 3Technological Innovation Center for Biological Control of Crop Diseases and Insect Pests of Hebei Province, Baoding 071001, China Published Online:10 Jan 2022https://doi.org/10.1094/PDIS-06-21-1269-PDNAboutSectionsView articlePDFSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat View articleIn July 2020, potato plants (cv. Xisen 6) showing characteristic symptoms of aerial stem rot were observed in a field in Fengning Manchu Autonomous County, Chengde, Hebei Province (northern China). The disease incidence in that field (5 ha in size) was more than 50%. Aerial stem rot of potato has increased in prevalence over recent years in Chengde, and it can cause significant yield loss on susceptible cultivars such as Xisen 6 and Huangxin 226. Affected stem (light brown and water-soaked stem sections) pieces ∼0.5 cm in length were surface sterilized by dipping them in 75% ethanol for 1 min followed by three successive rinses with sterile distilled water. Then, the tissues were soaked in 200 µl of 0.9% saline for 20 min. Aliquots (20 μl) of three 10-fold dilutions of the tissue specimen soaking solution were plated onto crystal violet pectate (CVP) medium. The CVP plates were incubated at 28°C for 48 h. Colonies producing pits were restreaked and purified on Luria-Bertani agar plates. The bacterial gDNA was extracted using the EasyPure Bacteria Genomic DNA Kit (TransGen Biotech, Beijing, China). The 16S rDNA region was amplified by PCR using the universal primers 27F/1492R (Weisburg et al. 1991) and sequenced. Results of the BLASTn analysis of the 16S rDNA amplicons (MZ348607, MZ348608) suggested that the isolates FN20211 and FN20222 belonged to the genus Pectobacterium. Housekeeping genes including acnA, gapA, icdA, mdh, proA, and rpoS were also amplified using a set of primers (Ma et al. 2007; Waleron et al. 2008) followed by sequencing (MZ356250 to MZ356261). To determine the species of the stem rot Pectobacterium isolates, multilocus sequence analysis (MLSA) was performed with six housekeeping genes, and a phylogenetic tree was reconstructed using RAxML (https://github.com/stamatak/standard-RAxML). No sequence variation was observed at any MLSA locus between FN20211 and FN20222. The result of phylogenetic analysis showed that the isolates clustered with Pectobacterium polaris type strain NIBIO1006T, which was isolated from potato (Dees et al. 2017). And the concatenated sequence of the six loci of isolate FN20211/FN20222 is 100% identical to those of the strains PZ1 (CP046377.1) and WBC1 (GCF_011378945.1), which were isolated from potato in South Korea and from Chinese cabbage in China, respectively. Potato seedlings (cv. Xisen 6 and Favorita) were inoculated with the isolates FN20211 and FN20222 by injecting 100 µl of bacterial suspensions (108 CFU/ml) into the upper parts of the stems of potato plants, or injecting with 100 µl of 0.9% saline as a control. The seedlings were grown at 25°C and 50% relative humidity. Three days after inoculation, only the bacteria-inoculated seedlings showed disease symptoms resembling those observed in the field. Bacterial colonies were obtained from the infected stems and were identified using the same PCR primers as described above. Therefore, P. polaris isolates FN20211 and FN20222 fulfill Koch’s postulates for aerial stem rot of potato. P. polaris causing blackleg and soft rot on potato plants has been reported in European countries including Netherlands, Norway (Dees et al. 2017), and Poland (Waleron et al. 2019) and also in Pakistan (Sarfraz et al. 2019) and Russia (Voronina et al. 2021). To our knowledge, this is the first report of P. polaris causing aerial stem rot of potato in China. The stem rot poses a significant threat to the local potato industry, and further research on epidemiology and disease management options is needed.The author(s) declare no conflict of interest.References:Dees, M. W., et al. 2017. Int. J. Syst. Evol. Microbiol. 67:5222. https://doi.org/10.1099/ijsem.0.002448 Crossref, ISI, Google ScholarMa, B., et al. 2007. Phytopathology 97:1150. https://doi.org/10.1094/PHYTO-97-9-1150 Link, ISI, Google ScholarSarfraz, S., et al. 2019. Plant Dis. 103:1405. https://doi.org/10.1094/PDIS-08-18-1450-PDN Link, ISI, Google ScholarVoronina, M. V., et al. 2021. Plant Dis. 105:1851. https://doi.org/10.1094/PDIS-09-20-1864-PDN Link, Google ScholarWaleron, M., et al. 2008. Eur. J. Plant Pathol. 121:161. https://doi.org/10.1007/s10658-007-9260-3 Crossref, ISI, Google ScholarWaleron, M., et al. 2019. Plant Dis. 103:1409. https://doi.org/10.1094/PDIS-10-18-1849-PDN Link, Google ScholarWeisburg, W. G., et al. 1991. J. Bacteriol. 173:697. https://doi.org/10.1128/jb.173.2.697-703.1991 Crossref, ISI, Google ScholarJ. Wang and W. Han contributed equally to this research.Funding: This research work was supported by China Agriculture Research System of MOF and MARA.The author(s) declare no conflict of interest.DetailsFiguresLiterature CitedRelated Vol. 106, No. 2 February 2022SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionWater-soaked lesions on bougainvillea caused by Robbsia andropogonis (O. Morales-Galván et al.). Photo credit: L. F. Flores-López. Soybean leaf showing crinkling and downward curling, characteristic of infection by soybean mosaic virus (SMV) (S. van Bentum et al.). Photo credit: S. van Bentum. Metrics Downloaded 627 times Article History Issue Date: 28 Feb 2022Published: 10 Jan 2022First Look: 19 Aug 2021Accepted: 17 Aug 2021 Page: 755 Information© 2022 The American Phytopathological SocietyFundingChina Agriculture Research System of MOF and MARAKeywordspotatoPectobacterium polarisstem rotThe author(s) declare no conflict of interest.PDF downloadCited ByFirst Report of Pectobacterium punjabense Causing Blackleg and Soft Rot on Potato in Hebei and Fujian Province, ChinaU. Handique, Y. Cao, D. Wang, R. Zhang, W. Li, Q. Sun, Z. Feng, and J. Wu20 June 2022 | Plant Disease, Vol. 106, No. 7High-Quality Complete Genome Resource of Pectobacterium parvum Isolate FN20211 Causing Aerial Stem Rot of PotatoJinhui Wang, Wanxin Han, Minna Pirhonen, Yang Pan, Dai Zhang, Dongmei Zhao, Qian Li, Jianing Cheng, Zhihui Yang, and Jiehua Zhu1 May 2022 | Molecular Plant-Microbe Interactions, Vol. 35, No. 6Full Issue PDF20 May 2022 | Molecular Plant-Microbe Interactions, Vol. 35, No. 6Pectobacterium spp. isolated from rotting carrots obtained from markets in Gampaha district, Sri Lanka exhibit the potential of having broad host ranges23 May 2022 | European Journal of Plant Pathology, Vol. 68" @default.
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