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• W3192972805 abstract "Single-cell RNA-seq (scRNA-seq) profiles gene expression with high resolution. Here, we develop a stepwise computational method-called SCAPTURE to identify, evaluate, and quantify cleavage and polyadenylation sites (PASs) from 3' tag-based scRNA-seq. SCAPTURE detects PASs de novo in single cells with high sensitivity and accuracy, enabling detection of previously unannotated PASs. Quantified alternative PAS transcripts refine cell identity analysis beyond gene expression, enriching information extracted from scRNA-seq data. Using SCAPTURE, we show changes of PAS usage in PBMCs from infected versus healthy individuals at single-cell resolution." @default.
• W3192972805 created "2021-08-16" @default.
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• W3192972805 date "2021-08-10" @default.
• W3192972805 modified "2023-10-17" @default.
• W3192972805 title "SCAPTURE: a deep learning-embedded pipeline that captures polyadenylation information from 3′ tag-based RNA-seq of single cells" @default.
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• W3192972805 doi "https://doi.org/10.1186/s13059-021-02437-5" @default.
• W3192972805 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8353616" @default.
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• W3192972805 hasPublicationYear "2021" @default.
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