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- W3193173870 abstract "Carnitine palmitoyltransferase-1 (CPT-1) is a rate-controlling enzyme for long-chain fatty acid oxidation. This manuscript provides protocols for measuring CPT-1-mediated respiration in permeabilized, adherent cell monolayers and mitochondria freshly isolated from tissue, along with examples to assess the potency and specificity of interventions targeting CPT-1. Strengths of the approach include ease, speed, and breadth of analysis, whereas drawbacks include loss of physiological regulation in reductionist systems and indirect assessment of CPT-1 enzymatic activity. For complete details on the use and execution of this protocol, please refer to Divakaruni et al. (2018)." @default.
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- W3193173870 date "2021-09-01" @default.
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- W3193173870 title "Measuring CPT-1-mediated respiration in permeabilized cells and isolated mitochondria" @default.
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- W3193173870 doi "https://doi.org/10.1016/j.xpro.2021.100687" @default.
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