Matches in SemOpenAlex for { <https://semopenalex.org/work/W3193286140> ?p ?o ?g. }
- W3193286140 abstract "Abstract The limited sensitivity of Förster Resonance Energy Transfer (FRET) biosensors hinders their broader applications. Here, we develop an approach integrating high-throughput FRET sorting and next-generation sequencing (FRET-Seq) to identify sensitive biosensors with varying substrate sequences from large-scale libraries directly in mammalian cells, utilizing the design of self-activating FRET (saFRET) biosensor. The resulting biosensors of Fyn and ZAP70 kinases exhibit enhanced performance and enable the dynamic imaging of T-cell activation mediated by T cell receptor (TCR) or chimeric antigen receptor (CAR), revealing a highly organized ZAP70 subcellular activity pattern upon TCR but not CAR engagement. The ZAP70 biosensor elucidates the role of immunoreceptor tyrosine-based activation motif (ITAM) in affecting ZAP70 activation to regulate CAR functions. A saFRET biosensor-based high-throughput drug screening (saFRET-HTDS) assay further enables the identification of an FDA-approved cancer drug, Sunitinib, that can be repurposed to inhibit ZAP70 activity and autoimmune-disease-related T-cell activation." @default.
- W3193286140 created "2021-08-30" @default.
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- W3193286140 date "2021-08-19" @default.
- W3193286140 modified "2023-09-23" @default.
- W3193286140 title "Integration of FRET and sequencing to engineer kinase biosensors from mammalian cell libraries" @default.
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- W3193286140 doi "https://doi.org/10.1038/s41467-021-25323-x" @default.
- W3193286140 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8376904" @default.
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- W3193286140 hasPublicationYear "2021" @default.
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