FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3193635119> ?p ?o ?g. }

• W3193635119 endingPage "e0255585" @default.
• W3193635119 startingPage "e0255585" @default.
• W3193635119 abstract "Background Gastric cancer (GC) is a multifactorial disease with high mortality. Anti-HER2 therapy is a promising strategy in GC treatment and trastuzumab was approved by FDA (Food and Drug Administration) as the first and the second line of treatment of the disease. Purpose The aim of the study was to examine the effectiveness of a combination of etoposide with trastuzumab or pertuzumab in AGS gastric cancer cells and breast cancer cells such as MCF-7, MDA-MB-231 and HCC1954. Methods and findings The cytotoxic effects of the tested compounds against gastric and breast cancer cells were checked by MTT (3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide) assay. The anti-proliferative potential was analyzed by the incorporation of [ 3 H]-thymidine into DNA. Fluorescent microscopy and flow cytometry was used to demonstrate the effect of the compounds on apoptosis. The mitochondrial membrane potential, and the activity of caspase-8 and caspase-9 were assessed. Autophagosomes and autolysosomes formation was checked by flow cytometry. The concentrations of Beclin-1, LC3A and LC3B were performed using ELISA. The expression of LC3A/B was also determined. The results from our study proved that the combination of etoposide with anti-HER2 antibodies was not cytotoxic against breast cancer cells, whereas the combination of etoposide with anti-HER2 antibodies decreased viability and DNA biosynthesis in gastric cancer cells. The interaction of etoposide with pertuzumab or trastuzumab induced programmed cell death via extrinsic and intrinsic apoptotic pathways in AGS gastric cancer cells, but did not affect autophagy, where a decrease of Beclin-1, LC3A and LC3B was observed in comparison with the untreated control. Conclusions The study demonstrated that etoposide (12.5 μM) with pertuzumab represent a promising strategy in gastric cancer treatment, but further in vivo examinations are also required." @default.
• W3193635119 created "2021-08-30" @default.
• W3193635119 creator A5018664072 @default.
• W3193635119 creator A5037505099 @default.
• W3193635119 creator A5042062147 @default.
• W3193635119 creator A5045766933 @default.
• W3193635119 creator A5087537524 @default.
• W3193635119 date "2021-08-26" @default.
• W3193635119 modified "2023-09-24" @default.
• W3193635119 title "Anti-HER2 monoclonal antibodies intensify the susceptibility of human gastric cancer cells to etoposide by promoting apoptosis, but not autophagy" @default.
• W3193635119 cites W1940768002 @default.
• W3193635119 cites W1985184275 @default.
• W3193635119 cites W1989078916 @default.
• W3193635119 cites W1993341852 @default.
• W3193635119 cites W2006483529 @default.
• W3193635119 cites W2035332337 @default.
• W3193635119 cites W2035484878 @default.
• W3193635119 cites W2040512733 @default.
• W3193635119 cites W2043968692 @default.
• W3193635119 cites W2046419550 @default.
• W3193635119 cites W2051907464 @default.
• W3193635119 cites W2055922116 @default.
• W3193635119 cites W2056274991 @default.
• W3193635119 cites W2064529883 @default.
• W3193635119 cites W2094627540 @default.
• W3193635119 cites W2099608239 @default.
• W3193635119 cites W2100439220 @default.
• W3193635119 cites W2104759721 @default.
• W3193635119 cites W2108182027 @default.
• W3193635119 cites W2126620646 @default.
• W3193635119 cites W2126968146 @default.
• W3193635119 cites W2141393790 @default.
• W3193635119 cites W2142911989 @default.
• W3193635119 cites W2144162491 @default.
• W3193635119 cites W2145510402 @default.
• W3193635119 cites W2165824128 @default.
• W3193635119 cites W2170016968 @default.
• W3193635119 cites W2253741745 @default.
• W3193635119 cites W2318605753 @default.
• W3193635119 cites W2515368941 @default.
• W3193635119 cites W2579589916 @default.
• W3193635119 cites W2598010207 @default.
• W3193635119 cites W2598685895 @default.
• W3193635119 cites W2600514678 @default.
• W3193635119 cites W2610367200 @default.
• W3193635119 cites W2779096670 @default.
• W3193635119 cites W2793972506 @default.
• W3193635119 cites W2892163061 @default.
• W3193635119 cites W2945117005 @default.
• W3193635119 cites W2982496779 @default.
• W3193635119 cites W3044218834 @default.
• W3193635119 cites W3115240252 @default.
• W3193635119 cites W3120114375 @default.
• W3193635119 cites W3135188769 @default.
• W3193635119 cites W854922956 @default.
• W3193635119 doi "https://doi.org/10.1371/journal.pone.0255585" @default.
• W3193635119 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8389407" @default.
• W3193635119 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34437575" @default.
• W3193635119 hasPublicationYear "2021" @default.
• W3193635119 type Work @default.
• W3193635119 sameAs 3193635119 @default.
• W3193635119 citedByCount "3" @default.
• W3193635119 countsByYear W31936351192022 @default.
• W3193635119 countsByYear W31936351192023 @default.
• W3193635119 crossrefType "journal-article" @default.
• W3193635119 hasAuthorship W3193635119A5018664072 @default.
• W3193635119 hasAuthorship W3193635119A5037505099 @default.
• W3193635119 hasAuthorship W3193635119A5042062147 @default.
• W3193635119 hasAuthorship W3193635119A5045766933 @default.
• W3193635119 hasAuthorship W3193635119A5087537524 @default.
• W3193635119 hasBestOaLocation W31936351191 @default.
• W3193635119 hasConcept C121608353 @default.
• W3193635119 hasConcept C126322002 @default.
• W3193635119 hasConcept C153911025 @default.
• W3193635119 hasConcept C154317977 @default.
• W3193635119 hasConcept C185592680 @default.
• W3193635119 hasConcept C190283241 @default.
• W3193635119 hasConcept C202751555 @default.
• W3193635119 hasConcept C2776694085 @default.
• W3193635119 hasConcept C2778119113 @default.
• W3193635119 hasConcept C2779786085 @default.
• W3193635119 hasConcept C2780783641 @default.
• W3193635119 hasConcept C502942594 @default.
• W3193635119 hasConcept C530470458 @default.
• W3193635119 hasConcept C553184892 @default.
• W3193635119 hasConcept C55493867 @default.
• W3193635119 hasConcept C71924100 @default.
• W3193635119 hasConcept C86803240 @default.
• W3193635119 hasConcept C96232424 @default.
• W3193635119 hasConceptScore W3193635119C121608353 @default.
• W3193635119 hasConceptScore W3193635119C126322002 @default.
• W3193635119 hasConceptScore W3193635119C153911025 @default.
• W3193635119 hasConceptScore W3193635119C154317977 @default.
• W3193635119 hasConceptScore W3193635119C185592680 @default.
• W3193635119 hasConceptScore W3193635119C190283241 @default.
• W3193635119 hasConceptScore W3193635119C202751555 @default.
• W3193635119 hasConceptScore W3193635119C2776694085 @default.
• W3193635119 hasConceptScore W3193635119C2778119113 @default.

• next