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• W3194120410 abstract "<ns4:p><ns4:bold>Background:</ns4:bold> Contributing factors for improved survival of human adipocytes mesenchymal stem cells (h-AMSCs) cultured through hypoxia preconditioning, in example apoptosis inhibition involving BCL2 and HSP27 expression, trigger signal expression (VEGF), SCF expression, OCT-4 expression, and CD44+ expression. The objective if this study was to explain the mechanism and role of hypoxic preconditioning and the optimal duration of hypoxic preconditioning exposure to improve survival of h-AMSCs.</ns4:p><ns4:p> <ns4:bold>Methods:</ns4:bold> An experimental laboratory explorative study (<ns4:italic>in vitro</ns4:italic>) with hypoxic preconditioning in h-AMSCs cultures. This research was conducted through four stages. First, isolation of h-AMSCs culture from adipose tissue of patients. Second, the characterization of h-AMSCs from adipose tissue by phenotype (flowcytometry) through CD44+, CD90+ and CD45-expression before being pre-conditioned for hypoxic treatment. Third, the hypoxic preconditioning in h-AMSCs culture (<ns4:italic>in vitro</ns4:italic>) was performed with an oxygen concentration of 1% for 24, 48 and 72 hours. Fourth, observation of survival from h-AMSCs culture was tested on the role of CD44+, VEGF, SCF, OCT-4, BCL2, HSP27 with Flowcytometry and apoptotic inhibition by Tunnel Assay method.</ns4:p><ns4:p> <ns4:bold>Results:</ns4:bold> The result of regression test showed that time difference had an effect on VEGF expression (<ns4:italic>p</ns4:italic><0.001;<ns4:bold>β</ns4:bold>=-0.482) and hypoxia condition also influenced VEGF expression (<ns4:italic>p</ns4:italic><0.001;<ns4:bold>β</ns4:bold>=0.774). The result of path analysis showed that SCF had effect on OCT-4 expression (<ns4:italic>p</ns4:italic><0.001; <ns4:bold>β</ns4:bold>=0.985). The regression test results showed that time effects on HSP27 expression (<ns4:italic>p</ns4:italic><0.001; <ns4:bold>β</ns4:bold>=0.398) and hypoxia precondition also affects HSP27 expression (<ns4:italic>p</ns4:italic><0.001; <ns4:bold>β</ns4:bold>=0.847). Pathway analysis showed that BCL2 expression inhibited apoptosis (<ns4:italic>p</ns4:italic>=0.030; <ns4:bold>β</ns4:bold>=-0.442) and HSP27 expression also inhibited apoptosis (<ns4:italic>p</ns4:italic><0,001;<ns4:bold>β</ns4:bold>=-0.487).</ns4:p><ns4:p> <ns4:bold>Conclusion: </ns4:bold>Hypoxic preconditioning of h-AMSC culture has proven to increase the expression of VEGF, SCF, OCT-4, and BCL2 and HSP27. This study demonstrated and explained the existence of a new mechanism of increased h-AMSC survival in cultures with hypoxic preconditioning (O2 1%) via VEGF, SCF, OCT-4, BCL2, and HSP 27.</ns4:p>" @default.
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• W3194120410 date "2021-08-23" @default.
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• W3194120410 title "Hypoxic preconditioning promotes survival of human adipocyte mesenchymal stem cell via expression of prosurvival and proangiogenic biomarkers" @default.
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