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- W3194865402 endingPage "100109" @default.
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- W3194865402 abstract "Platelets promote tumor metastasis by inducing promalignant phenotypes in cancer cells and directly contributing to cancer-related thrombotic complications. Platelet-derived extracellular vesicles (EVs) can promote epithelial-mesenchymal transition (EMT) in cancer cells, which confers high-grade malignancy. 12S-hydroxyeicosatetraenoic acid (12-HETE) generated by platelet-type 12-lipoxygenase (12-LOX) is considered a key modulator of cancer metastasis through unknown mechanisms. In platelets, 12-HETE can be esterified into plasma membrane phospholipids (PLs), which drive thrombosis. Using cocultures of human platelets and human colon adenocarcinoma cells (line HT29) and LC-MS/MS, we investigated the impact of platelets on cancer cell biosynthesis of 12S-HETE and its esterification into PLs and whether platelet ability to transfer its molecular cargo might play a role. To this aim, we performed coculture experiments with CFSE[5-(and-6)-carboxyfluorescein diacetate, succinimidyl ester]-loaded platelets. HT29 cells did not generate 12S-HETE or express 12-LOX. However, they acquired the capacity to produce 12S-HETE mainly esterified in plasmalogen phospholipid forms following the uptake of platelet-derived medium-sized EVs (mEVs) expressing 12-LOX. 12-LOX was detected in plasma mEV of patients with adenomas/adenocarcinomas, implying their potential to deliver the protein to cancer cells in vivo. In cancer cells exposed to platelets, endogenous but not exogenous 12S-HETE contributed to changes in EMT gene expression, mitigated by three structurally unrelated 12-LOX inhibitors. In conclusion, we showed that platelets induce the generation of primarily esterified 12-HETE in colon cancer cells following mEV-mediated delivery of 12-LOX. The modification of cancer cell phospholipids by 12-HETE may functionally impact cancer cell biology and represent a novel target for anticancer agent development." @default.
- W3194865402 created "2021-08-30" @default.
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- W3194865402 date "2021-01-01" @default.
- W3194865402 modified "2023-10-12" @default.
- W3194865402 title "Platelets induce free and phospholipid-esterified 12-hydroxyeicosatetraenoic acid generation in colon cancer cells by delivering 12-lipoxygenase" @default.
- W3194865402 cites W145418268 @default.
- W3194865402 cites W1529507327 @default.
- W3194865402 cites W1579531 @default.
- W3194865402 cites W1955468565 @default.
- W3194865402 cites W1964847767 @default.
- W3194865402 cites W1966419117 @default.
- W3194865402 cites W1968519475 @default.
- W3194865402 cites W1979291608 @default.
- W3194865402 cites W1985787110 @default.
- W3194865402 cites W1987168967 @default.
- W3194865402 cites W1989366241 @default.
- W3194865402 cites W1997241120 @default.
- W3194865402 cites W1998599956 @default.
- W3194865402 cites W2009109415 @default.
- W3194865402 cites W2012063163 @default.
- W3194865402 cites W2020037239 @default.
- W3194865402 cites W2021446146 @default.
- W3194865402 cites W2039387432 @default.
- W3194865402 cites W2039716262 @default.
- W3194865402 cites W2041830158 @default.
- W3194865402 cites W2044280391 @default.
- W3194865402 cites W2047821043 @default.
- W3194865402 cites W2048540822 @default.
- W3194865402 cites W2062695729 @default.
- W3194865402 cites W2064766999 @default.
- W3194865402 cites W2069697132 @default.
- W3194865402 cites W2074459205 @default.
- W3194865402 cites W2080828541 @default.
- W3194865402 cites W2083332835 @default.
- W3194865402 cites W2084997952 @default.
- W3194865402 cites W2104713951 @default.
- W3194865402 cites W2106489008 @default.
- W3194865402 cites W2111695773 @default.
- W3194865402 cites W2117692326 @default.
- W3194865402 cites W2124339684 @default.
- W3194865402 cites W2124422993 @default.
- W3194865402 cites W2126722127 @default.
- W3194865402 cites W2127702161 @default.
- W3194865402 cites W2139126860 @default.
- W3194865402 cites W2144581717 @default.
- W3194865402 cites W2154589460 @default.
- W3194865402 cites W2166629025 @default.
- W3194865402 cites W2170661234 @default.
- W3194865402 cites W2172260616 @default.
- W3194865402 cites W2265836483 @default.
- W3194865402 cites W2316741846 @default.
- W3194865402 cites W2321598543 @default.
- W3194865402 cites W2328750895 @default.
- W3194865402 cites W2412341010 @default.
- W3194865402 cites W2412409272 @default.
- W3194865402 cites W2593629244 @default.
- W3194865402 cites W2613670716 @default.
- W3194865402 cites W2613750599 @default.
- W3194865402 cites W2623360816 @default.
- W3194865402 cites W2753382806 @default.
- W3194865402 cites W2766110248 @default.
- W3194865402 cites W2770379522 @default.
- W3194865402 cites W2781800349 @default.
- W3194865402 cites W2789502632 @default.
- W3194865402 cites W2793109270 @default.
- W3194865402 cites W2793947839 @default.
- W3194865402 cites W2800789084 @default.
- W3194865402 cites W2806134841 @default.
- W3194865402 cites W2809887713 @default.
- W3194865402 cites W2858639613 @default.
- W3194865402 cites W2893778318 @default.
- W3194865402 cites W2901311599 @default.
- W3194865402 cites W2911417841 @default.
- W3194865402 cites W2914804288 @default.
- W3194865402 cites W2972952556 @default.
- W3194865402 cites W3037136069 @default.
- W3194865402 cites W3081509543 @default.
- W3194865402 cites W3118244472 @default.
- W3194865402 cites W844791963 @default.
- W3194865402 cites W2183558085 @default.
- W3194865402 doi "https://doi.org/10.1016/j.jlr.2021.100109" @default.
- W3194865402 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8456051" @default.
- W3194865402 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34428433" @default.