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- W3194965105 abstract "Abstract Adult neurogenesis has been described in two canonical regions of the adult central nervous system (CNS) of rodents, the subgranular zone (SGZ) of the hippocampus and the subependymal zone (SEZ) of the lateral ventricles. The stem cell niche of the SEZ provides a privileged environment composed of a specialized extracellular matrix (ECM) that comprises the glycoproteins tenascin-C (Tnc) and laminin-1 (LN1). In the present study, we investigated the function of these ECM glycoproteins in the adult stem cell niche. Adult neural stem cells (aNSPCs) of the SEZ were prepared from wildtype ( Tnc +/+ ) and Tnc knockout ( Tnc -/- ) mice and analyzed using molecular and cell biological approaches. A delayed maturation of aNSPCs in Tnc -/- tissue was reflected by a reduced capacity to form neurospheres in response to epidermal growth factor (EGF). In order to examine a potential influence of the ECM on cell proliferation, aNSPCs of both genotypes were studied by cell tracking using digital video microscopy. aNSPCs were cultivated on three different substrates, namely poly-D-Lysine (PDL) and PDL replenished with either LN1 or Tnc for up to six days in vitro. On each of the three substrates aNSPCs displayed lineage trees that could be investigated with regard to cell cycle length. The latter appeared reduced in Tnc -/- aNSPCs on PDL and LN1 substrates, less so on Tnc that seemed to compensate the absence of the ECM compound to some extent. Close inspection of the lineage trees revealed a subpopulation of late dividing aNSPCs late that engaged into cycling after a notable delay. aNSPCs late exhibited a clearly different morphology, with a larger cell body and conspicuous processes. aNSPCs late reiterated the reduction in cell cycle length on all substrates tested, which was not rescued on Tnc substrates. When the migratory activity of aNSPC-derived progeny was determined, Tnc -/- neuroblasts displayed significantly longer migration tracks. This was traced to an increased rate of migration episodes compared to the wildtype cells that rested for longer time periods. We conclude that Tnc intervenes in the proliferation of aNSPCs and modulates the motility of neuroblasts in the niche of the SEZ." @default.
- W3194965105 created "2021-08-30" @default.
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- W3194965105 date "2021-07-15" @default.
- W3194965105 modified "2023-10-17" @default.
- W3194965105 title "Cell Tracking of Adult Neural Stem Cells From The Subependymal Zone In Vitro Reveals Cell Type-Specific Impacts On Cell Cycle Progression By The Extracellular Matrix Molecule Tenascin-C" @default.
- W3194965105 doi "https://doi.org/10.21203/rs.3.rs-679173/v1" @default.
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