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- W3194977654 abstract "Abstract We demonstrate a versatile single‐laser platform for single‐beam dual‐color two‐photon spectroscopy that combines a short‐pulse laser source with a tunable broadband wavelength converter based on a highly nonlinear photonic‐crystal fiber (PCF). We show that the short‐pulse PCF output can be tailored, via dispersion and nonlinearity management, to deliver a broadband optical driver whose spectral structure is ideally suited for a single‐beam two‐photon absorption (2PA) spectroscopy of the next‐generation genetically encodable fluorescent‐protein (FP) sensors of pH and redox‐responsive contrast agents. As a promising spectroscopic resource for redox biology, the short‐pulse PCF output can be finely sculpted to alternately drive an FP system via a one of its two 2PA‐allowed quantum pathways, yielding a high‐contrast fluorescence readout for a highly sensitive detection of redox reactions and signaling, pH sensing, and oxidative‐stress diagnosis." @default.
- W3194977654 created "2021-08-30" @default.
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- W3194977654 date "2021-08-26" @default.
- W3194977654 modified "2023-10-18" @default.
- W3194977654 title "Single‐beam dual‐color alternate‐pathway two‐photon spectroscopy: Toward an optical toolbox for redox biology" @default.
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- W3194977654 doi "https://doi.org/10.1002/jrs.6183" @default.
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