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• W3194982815 endingPage "737367" @default.
• W3194982815 startingPage "737367" @default.
• W3194982815 abstract "Copper (Cu) is a common contaminant in water and can potentially cause hepatic tissue damage and toxicity in aquatic organisms. Herein we performed transcriptome analysis to investigate the effects of 100 μg/L and 400 μg/L Cu2+ (96-h exposure) on hepatic lipid metabolism in swamp eel (Monopterus albus). We identified 1004 and 2766 differentially expressed genes (DEGs) in the 100 and 400 μg/L Cu2+ groups, respectively. 4 up-regulated DEGs (e.g. DGAT2 and SRD5A1) and 25 down-regulated DEGs related with lipid metabolism (e.g. FABP7A, CYP51, FADS2 and ACAA2) were found in 100 μg/L Cu2+ group. In 400 μg/L Cu2+ group, 52 up-regulated lipid metabolism genes (e.g. PPARα, RXRα, ACSL4A, CPT-1, CPT-2, ACOX3, HADHA and ACADL) were identified, and 22 genes related with lipd metabolism including FABP7A, CYP51, CYP27B1, etc. were significantly down-regulated (p < 0.05). Gene ontology (GO) term and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses revealed that low concentrations of Cu (100 μg/L Cu2+) affected only few pathways, however, high concentrations of Cu (400 μg/L Cu2+) significantly affected more pathways, and peroxisome proliferator-activated receptor signaling and fatty acid degradation pathways were simultaneously in both two groups. Furthermore, upon alternative splicing analysis, we identified 55 and 81 differentially alternatively spliced (DAS) genes in the low and high concentration groups, respectively. Among these DAS genes, ACACA, DEGS2 and ST3GAL3 were associated with lipid metabolic process and fatty acid biosynthesis. The expression levels of 11 DEGs related to fatty acid degradation were validated by quantitative real-time PCR with significant up-regulation in 400 μg/L Cu2+ group, and the DAS gene of skipping exon 29 in ACACA which involved in fatty acid biosynthesis was validated by semiquantitative PCR. These results suggest that high Cu concentration (400 μg/L Cu2+) might reduce fatty acid deposition in swamp eel livers. To summarize, the genes and pathways identified herein should further our understanding of the mechanisms underlying Cu-induced lipid metabolism disorder. Furthermore, the present study highlights the importance of alternative splicing analysis, in tandem with gene expression surveys, to characterize molecular pathways in environmental studies." @default.
• W3194982815 created "2021-08-30" @default.
• W3194982815 creator A5014802136 @default.
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• W3194982815 creator A5065393214 @default.
• W3194982815 date "2022-01-01" @default.
• W3194982815 modified "2023-09-23" @default.
• W3194982815 title "Integrative analyses of gene expression and alternative splicing to gain insights into the effects of copper on hepatic lipid metabolism in swamp eel (Monopterus albus)" @default.
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• W3194982815 doi "https://doi.org/10.1016/j.aquaculture.2021.737367" @default.
• W3194982815 hasPublicationYear "2022" @default.
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