FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3195207979> ?p ?o ?g. }

• W3195207979 abstract "The toxicity and disposal concerns of organic solvents used in conventional extraction purposes has entailed the need for greener alternatives. Among such techniques, supercritical fluid extraction (SFE) has gained popularity by yielding extracts of high purity in a much faster manner. Carbon dioxide (CO 2 ) is generally preferred as a supercritical solvent because of its lower temperature requirements, better diffusivity and easy removal. The present study describes the characterization of supercritical CO 2 extracts of Indian variety of Cordyceps sinensis (CS)- a high-altitude medicinal mushroom widely revered in traditional medicine for its extensive anti-hypercholesterolemic, anti-inflammatory, anti-proliferative and energy-enhancing properties. Experimental parameters viz. 300 and 350 bar of extraction pressure, 60°C of temperature, 0.4°L/h CO 2 of flow rate and use of 1% (v/v) of ethanol as entrainer were optimized to prepare three different extracts namely, CSF1, CSF2 and CSF3. High-performance thin-layer chromatography (HPTLC) was used for assessing the quality of all the extracts in terms of cordycepin, the pivot biomarker compound in CS. Characterization by HPTLC and GC-MS confirmed the presence of flavonoids and nucleobases and, volatile organic compounds (VOCs), respectively. The chromatographic data acquired from metabolite profiling were subjected to chemometric analysis in an open source R studio which illustrated interrelatedness between CSF1 and CSF2 in terms of two major principal components. i.e. Dim 1 and Dim 2 whose values were 40.33 and 30.52% in variables factor map plotted using the HPTLC-generated retardation factor values. The factor maps based on retention times of the VOCs exhibited a variance of Dim 1 = 43.95% and Dim 2 = 24.85%. Furthermore, the extracts demonstrated appreciable antibacterial activity against Escherichia coli and Salmonella typhi by generation of reactive oxygen species (ROS), protein leakage and efflux pump inhibition within bacterial pathogens. CSFs were elucidated to be significantly cytoprotective ( p &lt; 0.05) in a simulated hypobaric hypoxia milieu (0.5% oxygen). CSF2 showed the best results by effectively improving the viability of human embryonic kidney (HEK 293) cells to 82.36 ± 1.76% at an optimum dose of 100 µg/ml. Levels of hypoxia inducible factor-1 alpha (HIF-1α) were modulated four-fold upon supplementation with CSF2. The results collectively evinced that the CSF extracts are substantially bioactive and could be effectively utilized as mycotherapeutics for multiple bioeffects." @default.
• W3195207979 created "2021-08-30" @default.
• W3195207979 creator A5041292243 @default.
• W3195207979 creator A5051676995 @default.
• W3195207979 creator A5064585212 @default.
• W3195207979 creator A5066972805 @default.
• W3195207979 date "2021-08-26" @default.
• W3195207979 modified "2023-09-26" @default.
• W3195207979 title "Supercritical Carbon Dioxide Extracts of Cordyceps sinensis: Chromatography-based Metabolite Profiling and Protective Efficacy Against Hypobaric Hypoxia" @default.
• W3195207979 cites W1520145079 @default.
• W3195207979 cites W1623195445 @default.
• W3195207979 cites W1810377575 @default.
• W3195207979 cites W1867098772 @default.
• W3195207979 cites W1903029975 @default.
• W3195207979 cites W1908444152 @default.
• W3195207979 cites W1920475760 @default.
• W3195207979 cites W1944495115 @default.
• W3195207979 cites W1971352565 @default.
• W3195207979 cites W1972528245 @default.
• W3195207979 cites W1978861666 @default.
• W3195207979 cites W1998636310 @default.
• W3195207979 cites W1998963478 @default.
• W3195207979 cites W2004579693 @default.
• W3195207979 cites W2007524018 @default.
• W3195207979 cites W2008291064 @default.
• W3195207979 cites W2010733552 @default.
• W3195207979 cites W2013937589 @default.
• W3195207979 cites W2020508907 @default.
• W3195207979 cites W2022500847 @default.
• W3195207979 cites W2033736337 @default.
• W3195207979 cites W2042510247 @default.
• W3195207979 cites W2044878397 @default.
• W3195207979 cites W2048445905 @default.
• W3195207979 cites W2051803873 @default.
• W3195207979 cites W2059024020 @default.
• W3195207979 cites W2059253191 @default.
• W3195207979 cites W2059299431 @default.
• W3195207979 cites W2059341245 @default.
• W3195207979 cites W2062775668 @default.
• W3195207979 cites W2067005914 @default.
• W3195207979 cites W2070682733 @default.
• W3195207979 cites W2074473690 @default.
• W3195207979 cites W2078748737 @default.
• W3195207979 cites W2079738059 @default.
• W3195207979 cites W2082142840 @default.
• W3195207979 cites W2084544115 @default.
• W3195207979 cites W2092994821 @default.
• W3195207979 cites W2096183100 @default.
• W3195207979 cites W2111646717 @default.
• W3195207979 cites W2114918609 @default.
• W3195207979 cites W2116373960 @default.
• W3195207979 cites W2123479846 @default.
• W3195207979 cites W2124820238 @default.
• W3195207979 cites W2126203280 @default.
• W3195207979 cites W2144868623 @default.
• W3195207979 cites W2147116464 @default.
• W3195207979 cites W2149504196 @default.
• W3195207979 cites W2154008318 @default.
• W3195207979 cites W2191349266 @default.
• W3195207979 cites W2220472210 @default.
• W3195207979 cites W2263191556 @default.
• W3195207979 cites W2290208379 @default.
• W3195207979 cites W2293168639 @default.
• W3195207979 cites W2342733468 @default.
• W3195207979 cites W2401243977 @default.
• W3195207979 cites W2480558262 @default.
• W3195207979 cites W2508162794 @default.
• W3195207979 cites W2522382278 @default.
• W3195207979 cites W2553010071 @default.
• W3195207979 cites W2562607567 @default.
• W3195207979 cites W2608530795 @default.
• W3195207979 cites W2734702968 @default.
• W3195207979 cites W2739776730 @default.
• W3195207979 cites W2744010562 @default.
• W3195207979 cites W2767642258 @default.
• W3195207979 cites W2767731763 @default.
• W3195207979 cites W2780857195 @default.
• W3195207979 cites W2790145885 @default.
• W3195207979 cites W2806601222 @default.
• W3195207979 cites W2884588730 @default.
• W3195207979 cites W2905138877 @default.
• W3195207979 cites W2929580203 @default.
• W3195207979 cites W2999575231 @default.
• W3195207979 cites W3015132278 @default.
• W3195207979 cites W3097793660 @default.
• W3195207979 cites W3120937497 @default.
• W3195207979 cites W3126840400 @default.
• W3195207979 cites W4251525979 @default.
• W3195207979 cites W61413653 @default.
• W3195207979 doi "https://doi.org/10.3389/fphar.2021.628924" @default.
• W3195207979 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8426348" @default.
• W3195207979 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34512317" @default.
• W3195207979 hasPublicationYear "2021" @default.
• W3195207979 type Work @default.
• W3195207979 sameAs 3195207979 @default.
• W3195207979 citedByCount "2" @default.
• W3195207979 countsByYear W31952079792022 @default.
• W3195207979 countsByYear W31952079792023 @default.
• W3195207979 crossrefType "journal-article" @default.
• W3195207979 hasAuthorship W3195207979A5041292243 @default.

• next