Matches in SemOpenAlex for { <https://semopenalex.org/work/W3195224616> ?p ?o ?g. }
- W3195224616 endingPage "1511" @default.
- W3195224616 startingPage "1497" @default.
- W3195224616 abstract "Understanding transcriptomes requires documenting the structures, modifications, and abundances of RNAs as well as their proximity to other molecules. The methods that make this possible depend critically on enzymes (including mutant derivatives) that act on nucleic acids for capturing and sequencing RNA. We tested two 3′ nucleotidyl transferases, Saccharomyces cerevisiae poly(A) polymerase and Schizosaccharomyces pombe Cid1, for the ability to add base and sugar modified rNTPs to free RNA 3′ ends, eventually focusing on Cid1. Although unable to polymerize ΨTP or 1meΨTP, Cid1 can use 5meUTP and 4thioUTP. Surprisingly, Cid1 can use inosine triphosphate to add poly(I) to the 3′ ends of a wide variety of RNA molecules. Most poly(A) mRNAs efficiently acquire a uniform tract of about 50 inosine residues from Cid1, whereas non-poly(A) RNAs acquire longer, more heterogeneous tails. Here we test these activities for use in direct RNA sequencing on nanopores, and find that Cid1-mediated poly(I)-tailing permits detection and quantification of both mRNAs and non-poly(A) RNAs simultaneously, as well as enabling the analysis of nascent RNAs associated with RNA polymerase II. Poly(I) produces a different current trace than poly(A), enabling recognition of native RNA 3′ end sequence lost by in vitro poly(A) addition. Addition of poly(I) by Cid1 offers a broadly useful alternative to poly(A) capture for direct RNA sequencing on nanopores." @default.
- W3195224616 created "2021-08-30" @default.
- W3195224616 creator A5012942178 @default.
- W3195224616 creator A5018121239 @default.
- W3195224616 creator A5044265645 @default.
- W3195224616 creator A5070071808 @default.
- W3195224616 creator A5073293462 @default.
- W3195224616 creator A5089867603 @default.
- W3195224616 date "2021-08-26" @default.
- W3195224616 modified "2023-10-16" @default.
- W3195224616 title "Synthesis of modified nucleotide polymers by the poly(U) polymerase Cid1: application to direct RNA sequencing on nanopores" @default.
- W3195224616 cites W1519765314 @default.
- W3195224616 cites W1966071984 @default.
- W3195224616 cites W1984859924 @default.
- W3195224616 cites W2020346499 @default.
- W3195224616 cites W2025836692 @default.
- W3195224616 cites W2028857610 @default.
- W3195224616 cites W2039169423 @default.
- W3195224616 cites W2040152367 @default.
- W3195224616 cites W2065548206 @default.
- W3195224616 cites W2073179720 @default.
- W3195224616 cites W2073581981 @default.
- W3195224616 cites W2083780833 @default.
- W3195224616 cites W2092017548 @default.
- W3195224616 cites W2104158537 @default.
- W3195224616 cites W2119560696 @default.
- W3195224616 cites W2127376800 @default.
- W3195224616 cites W2128769330 @default.
- W3195224616 cites W2136451426 @default.
- W3195224616 cites W2160912561 @default.
- W3195224616 cites W2305558625 @default.
- W3195224616 cites W2396522900 @default.
- W3195224616 cites W2503273054 @default.
- W3195224616 cites W2736038630 @default.
- W3195224616 cites W2767360874 @default.
- W3195224616 cites W2789843538 @default.
- W3195224616 cites W2801558310 @default.
- W3195224616 cites W2900214107 @default.
- W3195224616 cites W2900216082 @default.
- W3195224616 cites W2937096898 @default.
- W3195224616 cites W2949707636 @default.
- W3195224616 cites W2950063384 @default.
- W3195224616 cites W2950993016 @default.
- W3195224616 cites W2951278111 @default.
- W3195224616 cites W2953309432 @default.
- W3195224616 cites W2967438276 @default.
- W3195224616 cites W2982772605 @default.
- W3195224616 cites W2989499631 @default.
- W3195224616 cites W3026710232 @default.
- W3195224616 cites W3044566627 @default.
- W3195224616 cites W3122118726 @default.
- W3195224616 cites W3162353597 @default.
- W3195224616 cites W3165785950 @default.
- W3195224616 cites W4225743429 @default.
- W3195224616 doi "https://doi.org/10.1261/rna.078898.121" @default.
- W3195224616 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8594468" @default.
- W3195224616 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34446532" @default.
- W3195224616 hasPublicationYear "2021" @default.
- W3195224616 type Work @default.
- W3195224616 sameAs 3195224616 @default.
- W3195224616 citedByCount "9" @default.
- W3195224616 countsByYear W31952246162021 @default.
- W3195224616 countsByYear W31952246162022 @default.
- W3195224616 countsByYear W31952246162023 @default.
- W3195224616 crossrefType "journal-article" @default.
- W3195224616 hasAuthorship W3195224616A5012942178 @default.
- W3195224616 hasAuthorship W3195224616A5018121239 @default.
- W3195224616 hasAuthorship W3195224616A5044265645 @default.
- W3195224616 hasAuthorship W3195224616A5070071808 @default.
- W3195224616 hasAuthorship W3195224616A5073293462 @default.
- W3195224616 hasAuthorship W3195224616A5089867603 @default.
- W3195224616 hasBestOaLocation W31952246161 @default.
- W3195224616 hasConcept C104317684 @default.
- W3195224616 hasConcept C153911025 @default.
- W3195224616 hasConcept C181199279 @default.
- W3195224616 hasConcept C24107716 @default.
- W3195224616 hasConcept C2777610669 @default.
- W3195224616 hasConcept C552990157 @default.
- W3195224616 hasConcept C55493867 @default.
- W3195224616 hasConcept C67705224 @default.
- W3195224616 hasConcept C70721500 @default.
- W3195224616 hasConcept C82381507 @default.
- W3195224616 hasConcept C86803240 @default.
- W3195224616 hasConceptScore W3195224616C104317684 @default.
- W3195224616 hasConceptScore W3195224616C153911025 @default.
- W3195224616 hasConceptScore W3195224616C181199279 @default.
- W3195224616 hasConceptScore W3195224616C24107716 @default.
- W3195224616 hasConceptScore W3195224616C2777610669 @default.
- W3195224616 hasConceptScore W3195224616C552990157 @default.
- W3195224616 hasConceptScore W3195224616C55493867 @default.
- W3195224616 hasConceptScore W3195224616C67705224 @default.
- W3195224616 hasConceptScore W3195224616C70721500 @default.
- W3195224616 hasConceptScore W3195224616C82381507 @default.
- W3195224616 hasConceptScore W3195224616C86803240 @default.
- W3195224616 hasFunder F4320332161 @default.
- W3195224616 hasIssue "12" @default.
- W3195224616 hasLocation W31952246161 @default.
- W3195224616 hasLocation W31952246162 @default.