FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3195284687> ?p ?o ?g. }

• W3195284687 endingPage "114542" @default.
• W3195284687 startingPage "114542" @default.
• W3195284687 abstract "The root bark of Morus alba Linn. (M. alba), a traditional folk medicine, has been documented in the Chinese Pharmacopoeia, which has been widely used for asthma, fever, pneumonia, edema, vomit, colitis, bronchitis and keratitis diseases. Some of the diseases may be related to respiratory, digestive, urinary tract infections. Although Diels-Alder adducts (DAAs), flavonoids, 2-arylbenzofurans and stilbene compounds have been isolated from the root bark of M. alba, few compounds are reported for their antimicrobial efficacy in vivo and the mechanism.The aim of the study was to isolate and identify compounds of the root bark of M. alba in view of their anti-MRSA bioactivity, evaluate the anti-MRSA bioactivity of compounds and 60% ethanol elution (MA-6) in vitro and in vivo, and explore preliminary antibacterial mechanism in order to provide natural resources against MRSA infection.Systematic phytochemical investigations were carried out according to the thin layer chromatography (TLC) of the active fraction MA-6 to find more anti-MRSA ingredients. The compounds of the root bark of M. alba were separated by column chromatography and identified by LC-MS/MS and NMR spectroscopy. The anti-MRSA efficacy of the active ingredients were evaluated by broth microdilution method and a murine infection model. The mode of action of compounds was explored by time-kill curve and post-contact effect. The preliminary mechanism of compounds against MRSA was explored by drug efflux pumps and bacterial biofilms.Chemical isolation resulted in twenty-nine known compounds, most with one or more geranyl and prenyl units exhibited superior anti-MRSA bioactivity, with MIC values of 2-16 μg/mL. In addition, the mode of action indicated that compounds presented persistent antimicrobial effect, which also produced concentration-dependent and time-dependent killing activity or property. Preliminary mechanism showed that the compound kuwanon O (29) damaged the bacterial cell membranes, leading to the accumulation of antibiotics inside bacterial cells, moreover, MA-6 and kuwanon O (29) inhibited the efflux of drugs by combining with methicillin or ethidium bromide (EtBr), resulting in the MICs of EtBr and methicillin were obviously decreased three-fold. The anti-MRSA efficacy in vivo indicated that the active fraction MA-6 could reduce bacteria in spleen, liver, kidney and mortality of acutely infectious mice, which was better than the positive drug berberine chloride.Experimental investigation showed that the MA-6 and compound 29 have promising bioactivity against MRSA in vitro and in vivo, which might be used as a potential source of new antibacterial medicine or a potential efflux pump inhibitor against MRSA infection." @default.
• W3195284687 created "2021-08-30" @default.
• W3195284687 creator A5014388031 @default.
• W3195284687 creator A5015914278 @default.
• W3195284687 creator A5046133135 @default.
• W3195284687 creator A5059823306 @default.
• W3195284687 creator A5061579277 @default.
• W3195284687 creator A5066548463 @default.
• W3195284687 creator A5074400216 @default.
• W3195284687 creator A5075516445 @default.
• W3195284687 creator A5077940282 @default.
• W3195284687 creator A5086671763 @default.
• W3195284687 creator A5088751634 @default.
• W3195284687 date "2021-12-01" @default.
• W3195284687 modified "2023-10-01" @default.
• W3195284687 title "Bioguided isolation, identification and bioactivity evaluation of anti-MRSA constituents from Morus alba Linn." @default.
• W3195284687 cites W1531268983 @default.
• W3195284687 cites W1539470930 @default.
• W3195284687 cites W1647206315 @default.
• W3195284687 cites W1968634120 @default.
• W3195284687 cites W1973911317 @default.
• W3195284687 cites W1977605585 @default.
• W3195284687 cites W1984494327 @default.
• W3195284687 cites W1988027722 @default.
• W3195284687 cites W1991276906 @default.
• W3195284687 cites W1998015095 @default.
• W3195284687 cites W2000153201 @default.
• W3195284687 cites W2000812746 @default.
• W3195284687 cites W2006658612 @default.
• W3195284687 cites W2008134995 @default.
• W3195284687 cites W2014838100 @default.
• W3195284687 cites W2019173565 @default.
• W3195284687 cites W2020098805 @default.
• W3195284687 cites W2020913470 @default.
• W3195284687 cites W2021329131 @default.
• W3195284687 cites W2023093551 @default.
• W3195284687 cites W2023167979 @default.
• W3195284687 cites W2033178042 @default.
• W3195284687 cites W2036056925 @default.
• W3195284687 cites W2037166232 @default.
• W3195284687 cites W2041350348 @default.
• W3195284687 cites W2048611404 @default.
• W3195284687 cites W2054927411 @default.
• W3195284687 cites W2056965558 @default.
• W3195284687 cites W2064259049 @default.
• W3195284687 cites W2065511917 @default.
• W3195284687 cites W2070162605 @default.
• W3195284687 cites W2071939906 @default.
• W3195284687 cites W2074947213 @default.
• W3195284687 cites W2075589757 @default.
• W3195284687 cites W2076514771 @default.
• W3195284687 cites W2081331348 @default.
• W3195284687 cites W2082236880 @default.
• W3195284687 cites W2083963118 @default.
• W3195284687 cites W2090740701 @default.
• W3195284687 cites W2108896804 @default.
• W3195284687 cites W2121579679 @default.
• W3195284687 cites W2124712500 @default.
• W3195284687 cites W2136248634 @default.
• W3195284687 cites W2143801014 @default.
• W3195284687 cites W2324858000 @default.
• W3195284687 cites W2330564257 @default.
• W3195284687 cites W2331743104 @default.
• W3195284687 cites W2331795948 @default.
• W3195284687 cites W2466338161 @default.
• W3195284687 cites W2514024230 @default.
• W3195284687 cites W2516515667 @default.
• W3195284687 cites W2736292092 @default.
• W3195284687 cites W2766562044 @default.
• W3195284687 cites W2772143514 @default.
• W3195284687 cites W2775331525 @default.
• W3195284687 cites W2781087817 @default.
• W3195284687 cites W2810836489 @default.
• W3195284687 cites W2811121885 @default.
• W3195284687 cites W2888228594 @default.
• W3195284687 cites W2899146544 @default.
• W3195284687 cites W2912530939 @default.
• W3195284687 cites W2938274610 @default.
• W3195284687 cites W2940117744 @default.
• W3195284687 cites W3039910445 @default.
• W3195284687 cites W3041604486 @default.
• W3195284687 cites W3042458774 @default.
• W3195284687 cites W3127410554 @default.
• W3195284687 cites W4243381856 @default.
• W3195284687 doi "https://doi.org/10.1016/j.jep.2021.114542" @default.
• W3195284687 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34428525" @default.
• W3195284687 hasPublicationYear "2021" @default.
• W3195284687 type Work @default.
• W3195284687 sameAs 3195284687 @default.
• W3195284687 citedByCount "8" @default.
• W3195284687 countsByYear W31952846872022 @default.
• W3195284687 countsByYear W31952846872023 @default.
• W3195284687 crossrefType "journal-article" @default.
• W3195284687 hasAuthorship W3195284687A5014388031 @default.
• W3195284687 hasAuthorship W3195284687A5015914278 @default.
• W3195284687 hasAuthorship W3195284687A5046133135 @default.
• W3195284687 hasAuthorship W3195284687A5059823306 @default.
• W3195284687 hasAuthorship W3195284687A5061579277 @default.

• next