FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3195403605> ?p ?o ?g. }

• W3195403605 abstract "Background: Snake venoms are composed of pharmacologically active proteins that are evolutionarily diverse, stable and specific to targets. Hence, venoms have been explored as a source of bioactive molecules in treating numerous diseases. Recent evidences suggest that snake venom proteins may affect the formation of new blood vessels. Excessive angiogenesis has been implicated in several pathologies including tumours, diabetic retinopathy, arthritis, inter alia. In the present study, we have examined the effects of P-I metalloproteinases isolated from Bothrops moojeni (BmMP-1) and Bothrops atrox (BaMP-1) and L-amino acid oxidases (LAAO) isolated from B. moojeni (BmLAAO) and B. atrox (BaLAAO) on biochemical and functional aspects of angiogenesis. Methods: P-I metalloproteinases and LAAO were purified from venom by molecular size exclusion and ion-exchange chromatography and subsequently confirmed using mass spectrometry. The P-I metalloproteinases were characterized by azocaseinolytic, fibrinogenolytic and gelatinase activity and LAAO activity was assessed by enzyme activity on L-amino acids. Influence of these proteins on apoptosis and cell cycle in endothelial cells was analysed by flow cytometry. The angiogenic activity was determined by in vitro 3D spheroid assay, Matrigel tube forming assay, and in vivo agarose plug transformation in mice. Results: P-I metalloproteinases exhibited azocaseinolytic activity, cleaved α and partially β chain of fibrinogen, and displayed catalytic activity on gelatin. LAAO showed differential activity on L-amino acids. Flow cytometry analysis indicated that both P-I metalloproteinases and LAAO arrested the cells in G0/G1 phase and further induced both necrosis and apoptosis in endothelial cells. In vitro, P-I metalloproteinases and LAAO exhibited significant anti-angiogenic properties in 3D spheroid and Matrigel models by reducing sprout outgrowth and tube formation. Using agarose plug transplants in mice harbouring P-I metalloproteinases and LAAO we demonstrated a marked disruption of vasculature at the periphery. Conclusion: Our research suggests that P-I metalloproteinases and LAAO exhibit anti-angiogenic properties in vitro and in vivo." @default.
• W3195403605 created "2021-08-30" @default.
• W3195403605 creator A5012227417 @default.
• W3195403605 creator A5053451010 @default.
• W3195403605 creator A5053852414 @default.
• W3195403605 creator A5056043920 @default.
• W3195403605 creator A5056885389 @default.
• W3195403605 creator A5069456684 @default.
• W3195403605 creator A5071602177 @default.
• W3195403605 creator A5079082852 @default.
• W3195403605 date "2021-01-01" @default.
• W3195403605 modified "2023-10-02" @default.
• W3195403605 title "P-I metalloproteinases and L-amino acid oxidases from Bothrops species inhibit angiogenesis" @default.
• W3195403605 cites W1012654403 @default.
• W3195403605 cites W1479397831 @default.
• W3195403605 cites W1586431716 @default.
• W3195403605 cites W1682086660 @default.
• W3195403605 cites W1888609755 @default.
• W3195403605 cites W1965239722 @default.
• W3195403605 cites W1969815685 @default.
• W3195403605 cites W1971104392 @default.
• W3195403605 cites W1974085504 @default.
• W3195403605 cites W1975391008 @default.
• W3195403605 cites W1975744071 @default.
• W3195403605 cites W1977229831 @default.
• W3195403605 cites W1978320447 @default.
• W3195403605 cites W1983481237 @default.
• W3195403605 cites W1985459379 @default.
• W3195403605 cites W1986441751 @default.
• W3195403605 cites W1989678388 @default.
• W3195403605 cites W1990815622 @default.
• W3195403605 cites W2000000541 @default.
• W3195403605 cites W2005746041 @default.
• W3195403605 cites W2016654210 @default.
• W3195403605 cites W2017283877 @default.
• W3195403605 cites W2020278215 @default.
• W3195403605 cites W2022329243 @default.
• W3195403605 cites W2034604903 @default.
• W3195403605 cites W2039072197 @default.
• W3195403605 cites W2042851144 @default.
• W3195403605 cites W2043033465 @default.
• W3195403605 cites W2044140319 @default.
• W3195403605 cites W2055310192 @default.
• W3195403605 cites W2058175284 @default.
• W3195403605 cites W2060147003 @default.
• W3195403605 cites W2062668153 @default.
• W3195403605 cites W2067336074 @default.
• W3195403605 cites W2068018909 @default.
• W3195403605 cites W2071320462 @default.
• W3195403605 cites W2073625132 @default.
• W3195403605 cites W2074066573 @default.
• W3195403605 cites W2076727314 @default.
• W3195403605 cites W2077627236 @default.
• W3195403605 cites W2081547052 @default.
• W3195403605 cites W2082734133 @default.
• W3195403605 cites W2084738263 @default.
• W3195403605 cites W2090117493 @default.
• W3195403605 cites W2092234462 @default.
• W3195403605 cites W2121055414 @default.
• W3195403605 cites W2125787292 @default.
• W3195403605 cites W2127242142 @default.
• W3195403605 cites W2144115015 @default.
• W3195403605 cites W2144576314 @default.
• W3195403605 cites W2148187441 @default.
• W3195403605 cites W2152944425 @default.
• W3195403605 cites W2170598539 @default.
• W3195403605 cites W2208861868 @default.
• W3195403605 cites W2301121950 @default.
• W3195403605 cites W2307799631 @default.
• W3195403605 cites W2314562788 @default.
• W3195403605 cites W2333570837 @default.
• W3195403605 cites W2430587401 @default.
• W3195403605 cites W2467863152 @default.
• W3195403605 cites W2548010401 @default.
• W3195403605 cites W2741582961 @default.
• W3195403605 cites W2771389242 @default.
• W3195403605 cites W2887108758 @default.
• W3195403605 cites W2900772909 @default.
• W3195403605 cites W2909772574 @default.
• W3195403605 cites W2914978457 @default.
• W3195403605 cites W2917173090 @default.
• W3195403605 cites W2951973322 @default.
• W3195403605 cites W2971460026 @default.
• W3195403605 cites W2997891042 @default.
• W3195403605 cites W3004485000 @default.
• W3195403605 cites W3045103008 @default.
• W3195403605 cites W3087292437 @default.
• W3195403605 cites W3113276280 @default.
• W3195403605 cites W3148794667 @default.
• W3195403605 cites W4232808932 @default.
• W3195403605 cites W4285719527 @default.
• W3195403605 cites W4376522565 @default.
• W3195403605 cites W1989192235 @default.
• W3195403605 doi "https://doi.org/10.1590/1678-9199-jvatitd-2020-0180" @default.
• W3195403605 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8381740" @default.
• W3195403605 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34471403" @default.
• W3195403605 hasPublicationYear "2021" @default.
• W3195403605 type Work @default.
• W3195403605 sameAs 3195403605 @default.
• W3195403605 citedByCount "5" @default.

• next