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- W3195492202 abstract "While current group I ribozymes use several distinct strategies to function under conditions of low Mg2+ concentration (≤ 3 mM), a deletion mutant of the Tetrahymena ribozyme (ΔP5 ribozyme) is virtually inactive with 3 mM Mg2+ due to removal of the large peripheral module, P5abc, supporting the active conformation of the core module. We investigated the molecular crowding effects of synthetic polyethylene glycols (PEGs) on the activity of the ΔP5 ribozyme. Among PEG molecules with different chain lengths, PEG600 improved the activity of the ΔP5 ribozyme most effectively in the presence of 3 mM Mg2+." @default.
- W3195492202 created "2021-08-30" @default.
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- W3195492202 date "2021-08-17" @default.
- W3195492202 modified "2023-09-24" @default.
- W3195492202 title "Effects of chain length of polyethylene glycol molecular crowders on a mutant Tetrahymena group I ribozyme lacking large peripheral module" @default.
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- W3195492202 doi "https://doi.org/10.1080/15257770.2021.1956531" @default.
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