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- W3195495653 abstract "3013 APITD1 is a novel gene in the neuroblastoma tumor suppressor candidate region on chromosome 1p36.2. Gene expression is down-regulated in primary neuroblastoma tumors from high-risk patients and cell growth is reduced by APITD1 overexpression. The purpose of this study was to determine the function of the encoded protein. A custom made antibody was used in Western blot analysis to detect proteins at the predicted molecular weight of 16 kDa. Purification by immunoprecipitation and mass spectrometry (MS/MS) analysis confirmed the identity of the Apitd1 protein. While expression of the APITD1 gene is relatively low in neuroblastoma cell lines compared to mRNA levels in a panel of normal tissues, the level of Apitd1 protein is not significantly reduced in neuroblastoma cell lines when compared to other cell lines. Apitd1 contains a predicted TFIID-31 domain, representing the TATA box-binding protein-associated factor, TAFII31, required for p53 mediated transcription activation. Although a large proportion of cells transfected with APITD1 enter apoptosis, protein levels of p53 and the p53 responsive genes Mdm2, p21WAF/CIP1 and Bax are unchanged by APITD1 overexpression, indicating that Apitd1 is not a factor required for p53-mediated transcription activation. The level of endogenous Apitd1 is correlated to p53 levels but not to expression of the anti-apoptotic protein Bcl2. This study also shows that the Apitd1 protein is preferably located to the nucleus, consistent with a recently published study, where Apitd1 was identified as a distal protein in the CENP-A centromeric complex. In conclusion, based on its cytogenetic location, 1p36.2, and its biological features in neuroblastoma tumors, APITD1 presents as a candidate neuroblastoma tumor suppressor gene. The encoded protein may be involved in transcriptional regulation of genes which control cell survival or it may be involved in chromosome alignment and segregation during mitosis. In this case, the absence of APITD1 expression in primary neuroblastoma tumors could cause errors of chromosome segregation which further leads to structural or numerical chromosomal aberrations." @default.
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- W3195495653 date "2008-05-01" @default.
- W3195495653 modified "2023-09-23" @default.
- W3195495653 title "Apitd1, a nuclear protein with a histone-fold superfamily domain induces apoptosis in neuroblastoma cells" @default.
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