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• W3195702410 abstract "Positive-strand RNA viruses induce the biogenesis of unique membranous organelles called viral replication organelles (VROs), which perform virus replication in infected cells. Tombusviruses have been shown to rewire cellular trafficking and metabolic pathways, remodel host membranes, and recruit multiple host factors to support viral replication. In this work, we demonstrate that tomato bushy stunt virus (TBSV) and the closely related carnation Italian ringspot virus (CIRV) usurp Rab7 small GTPase to facilitate building VROs in the surrogate host yeast and in plants. Depletion of Rab7 small GTPase, which is needed for late endosome and retromer biogenesis, strongly inhibits TBSV and CIRV replication in yeast and in planta. The viral p33 replication protein interacts with Rab7 small GTPase, which results in the relocalization of Rab7 into the large VROs. Similar to the depletion of Rab7, the deletion of either MON1 or CCZ1 heterodimeric GEFs (guanine nucleotide exchange factors) of Rab7 inhibited TBSV RNA replication in yeast. This suggests that the activated Rab7 has proviral functions. We show that the proviral function of Rab7 is to facilitate the recruitment of the retromer complex and the endosomal sorting nexin-BAR proteins into VROs. We demonstrate that TBSV p33-driven retargeting of Rab7 into VROs results in the delivery of several retromer cargos with proviral functions. These proteins include lipid enzymes, such as Vps34 PI3K (phosphatidylinositol 3-kinase), PI4Kα-like Stt4 phosphatidylinositol 4-kinase, and Psd2 phosphatidylserine decarboxylase. In summary, based on these and previous findings, we propose that subversion of Rab7 into VROs allows tombusviruses to reroute endocytic and recycling trafficking to support virus replication. IMPORTANCE The replication of positive-strand RNA viruses depends on the biogenesis of viral replication organelles (VROs). However, the formation of membranous VROs is not well understood yet. Using tombusviruses and the model host yeast, we discovered that the endosomal Rab7 small GTPase is critical for the formation of VROs. Interaction between Rab7 and the TBSV p33 replication protein leads to the recruitment of Rab7 into VROs. TBSV-driven usurping of Rab7 has proviral functions through facilitating the delivery of the co-opted retromer complex, sorting nexin-BAR proteins, and lipid enzymes into VROs to create an optimal milieu for virus replication. These results open up the possibility that controlling cellular Rab7 activities in infected cells could be a target for new antiviral strategies." @default.
• W3195702410 created "2021-08-30" @default.
• W3195702410 creator A5052382043 @default.
• W3195702410 creator A5063751755 @default.
• W3195702410 creator A5090380640 @default.
• W3195702410 date "2021-10-13" @default.
• W3195702410 modified "2023-09-26" @default.
• W3195702410 title "Tombusviruses Target a Major Crossroad in the Endocytic and Recycling Pathways via Co-opting Rab7 Small GTPase" @default.
• W3195702410 cites W11700218 @default.
• W3195702410 cites W1504404542 @default.
• W3195702410 cites W1564819673 @default.
• W3195702410 cites W1585380514 @default.
• W3195702410 cites W1704844374 @default.
• W3195702410 cites W1973836256 @default.
• W3195702410 cites W1979558190 @default.
• W3195702410 cites W1991400645 @default.
• W3195702410 cites W1995668581 @default.
• W3195702410 cites W1997385930 @default.
• W3195702410 cites W2006218543 @default.
• W3195702410 cites W2012885662 @default.
• W3195702410 cites W2022068951 @default.
• W3195702410 cites W2025003808 @default.
• W3195702410 cites W2027129957 @default.
• W3195702410 cites W2034388774 @default.
• W3195702410 cites W2044446306 @default.
• W3195702410 cites W2059854128 @default.
• W3195702410 cites W2060078754 @default.
• W3195702410 cites W2064627646 @default.
• W3195702410 cites W2074422749 @default.
• W3195702410 cites W2080000174 @default.
• W3195702410 cites W2083901349 @default.
• W3195702410 cites W2086067323 @default.
• W3195702410 cites W2088207308 @default.
• W3195702410 cites W2089400774 @default.
• W3195702410 cites W2095460902 @default.
• W3195702410 cites W2100067436 @default.
• W3195702410 cites W2107113045 @default.
• W3195702410 cites W2110533625 @default.
• W3195702410 cites W2117821184 @default.
• W3195702410 cites W2119477250 @default.
• W3195702410 cites W2119948288 @default.
• W3195702410 cites W2130090754 @default.
• W3195702410 cites W2135404101 @default.
• W3195702410 cites W2136058493 @default.
• W3195702410 cites W2137538896 @default.
• W3195702410 cites W2143210808 @default.
• W3195702410 cites W2153565955 @default.
• W3195702410 cites W2154046032 @default.
• W3195702410 cites W2155494439 @default.
• W3195702410 cites W2160321475 @default.
• W3195702410 cites W2161029268 @default.
• W3195702410 cites W2161932769 @default.
• W3195702410 cites W2167153850 @default.
• W3195702410 cites W2230135021 @default.
• W3195702410 cites W2255835837 @default.
• W3195702410 cites W2271346443 @default.
• W3195702410 cites W2300523604 @default.
• W3195702410 cites W2309638858 @default.
• W3195702410 cites W2316450091 @default.
• W3195702410 cites W2399567181 @default.
• W3195702410 cites W2414220668 @default.
• W3195702410 cites W2418339155 @default.
• W3195702410 cites W2426332364 @default.
• W3195702410 cites W2469926161 @default.
• W3195702410 cites W2475341895 @default.
• W3195702410 cites W2476126056 @default.
• W3195702410 cites W2511932783 @default.
• W3195702410 cites W2513814202 @default.
• W3195702410 cites W2533211970 @default.
• W3195702410 cites W2534368867 @default.
• W3195702410 cites W2546391660 @default.
• W3195702410 cites W2553944165 @default.
• W3195702410 cites W2556400981 @default.
• W3195702410 cites W2575593256 @default.
• W3195702410 cites W2578709657 @default.
• W3195702410 cites W2587718283 @default.
• W3195702410 cites W2680117374 @default.
• W3195702410 cites W2740138820 @default.
• W3195702410 cites W2761297154 @default.
• W3195702410 cites W2766507999 @default.
• W3195702410 cites W2766704353 @default.
• W3195702410 cites W2770032330 @default.
• W3195702410 cites W2784667865 @default.
• W3195702410 cites W2801448489 @default.
• W3195702410 cites W2805716732 @default.
• W3195702410 cites W2890632265 @default.
• W3195702410 cites W2895272505 @default.
• W3195702410 cites W2910016743 @default.
• W3195702410 cites W2912141593 @default.
• W3195702410 cites W2969230013 @default.
• W3195702410 cites W2977559159 @default.
• W3195702410 cites W2979674162 @default.
• W3195702410 cites W2981916477 @default.
• W3195702410 cites W3015373708 @default.
• W3195702410 cites W3032569113 @default.
• W3195702410 cites W3041282621 @default.
• W3195702410 cites W3085561100 @default.
• W3195702410 cites W3093900830 @default.
• W3195702410 cites W3096712778 @default.
• W3195702410 cites W3116773451 @default.

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