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- W3195750562 endingPage "9202" @default.
- W3195750562 startingPage "9202" @default.
- W3195750562 abstract "Aptamers feature a number of advantages, compared to antibodies. However, their application has been limited so far, mainly because of the complex selection process. 'High-throughput sequencing fluorescent ligand interaction profiling' (HiTS-FLIP) significantly increases the selection efficiency and is consequently a very powerful and versatile technology for the selection of high-performance aptamers. It is the first experiment to allow the direct and quantitative measurement of the affinity and specificity of millions of aptamers simultaneously by harnessing the potential of optical next-generation sequencing platforms to perform fluorescence-based binding assays on the clusters displayed on the flow cells and determining their sequence and position in regular high-throughput sequencing. Many variants of the experiment have been developed that allow automation and in situ conversion of DNA clusters into base-modified DNA, RNA, peptides, and even proteins. In addition, the information from mutational assays, performed with HiTS-FLIP, provides deep insights into the relationship between the sequence, structure, and function of aptamers. This enables a detailed understanding of the sequence-specific rules that determine affinity, and thus, supports the evolution of aptamers. Current variants of the HiTS-FLIP experiment and its application in the field of aptamer selection, characterisation, and optimisation are presented in this review." @default.
- W3195750562 created "2021-08-30" @default.
- W3195750562 creator A5006304159 @default.
- W3195750562 creator A5064393989 @default.
- W3195750562 date "2021-08-25" @default.
- W3195750562 modified "2023-10-16" @default.
- W3195750562 title "High-Throughput Selection and Characterisation of Aptamers on Optical Next-Generation Sequencers" @default.
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- W3195750562 doi "https://doi.org/10.3390/ijms22179202" @default.