FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3195852110> ?p ?o ?g. }

• W3195852110 endingPage "45" @default.
• W3195852110 startingPage "35" @default.
• W3195852110 abstract "The thousands of features commonly observed when performing untargeted metabolomics with quadrupole time-of-flight (QTOF) and Orbitrap mass spectrometers often correspond to only a few hundred unique metabolites of biological origin, which is in the range of what can be assayed in a single targeted metabolomics experiment by using a triple quadrupole (QqQ) mass spectrometer. A major benefit of performing targeted metabolomics with QqQ mass spectrometry is the affordability of the instruments relative to high-resolution QTOF and Orbitrap platforms. Optimizing targeted methods to profile hundreds of metabolites on a QqQ mass spectrometer, however, has historically been limited by the availability of authentic standards, particularly for “unknowns” that have yet to be structurally identified. Here, we report a strategy to develop multiple reaction monitoring (MRM) methods for QqQ instruments on the basis of high-resolution spectra, thereby enabling us to use data from untargeted metabolomics to design targeted experiments without the need for authentic standards. We demonstrate that using high-resolution fragmentation data alone to design MRM methods results in the same quantitative performance as when methods are optimized by measuring authentic standards on QqQ instruments, as is conventionally done. The approach was validated by showing that Orbitrap ID-X data can be used to establish MRM methods on a Thermo TSQ Altis and two Agilent QqQs for hundreds of metabolites, including unknowns, without a dependence on standards. Finally, we highlight an application where metabolite profiling was performed on an ID-X and a QqQ by using the strategy introduced here, with both data sets yielding the same result. The described approach therefore allows us to use QqQ instruments, which are often associated with targeted metabolomics, to profile knowns and unknowns at a comprehensive scale that is typical of untargeted metabolomics." @default.
• W3195852110 created "2021-08-30" @default.
• W3195852110 creator A5004347939 @default.
• W3195852110 creator A5006252438 @default.
• W3195852110 creator A5027513211 @default.
• W3195852110 creator A5044117588 @default.
• W3195852110 creator A5063652834 @default.
• W3195852110 creator A5085399017 @default.
• W3195852110 date "2021-07-22" @default.
• W3195852110 modified "2023-10-15" @default.
• W3195852110 title "A Workflow to Perform Targeted Metabolomics at the Untargeted Scale on a Triple Quadrupole Mass Spectrometer" @default.
• W3195852110 cites W143096088 @default.
• W3195852110 cites W1543068333 @default.
• W3195852110 cites W1611854178 @default.
• W3195852110 cites W1964010911 @default.
• W3195852110 cites W2013233148 @default.
• W3195852110 cites W2022067114 @default.
• W3195852110 cites W2022588068 @default.
• W3195852110 cites W2026896316 @default.
• W3195852110 cites W2028607242 @default.
• W3195852110 cites W2029742084 @default.
• W3195852110 cites W2079710791 @default.
• W3195852110 cites W2107710616 @default.
• W3195852110 cites W2114754207 @default.
• W3195852110 cites W2127360645 @default.
• W3195852110 cites W2155793022 @default.
• W3195852110 cites W2327371542 @default.
• W3195852110 cites W2518686384 @default.
• W3195852110 cites W254079993 @default.
• W3195852110 cites W2559284283 @default.
• W3195852110 cites W2560952263 @default.
• W3195852110 cites W2585101342 @default.
• W3195852110 cites W2725788675 @default.
• W3195852110 cites W2803865599 @default.
• W3195852110 cites W2806559730 @default.
• W3195852110 cites W2806920626 @default.
• W3195852110 cites W2886629339 @default.
• W3195852110 cites W2891937156 @default.
• W3195852110 cites W2906914952 @default.
• W3195852110 cites W2913666085 @default.
• W3195852110 cites W2962695977 @default.
• W3195852110 cites W2971068386 @default.
• W3195852110 cites W2974361058 @default.
• W3195852110 cites W2988351814 @default.
• W3195852110 cites W3003572520 @default.
• W3195852110 cites W3016036651 @default.
• W3195852110 cites W3037865744 @default.
• W3195852110 cites W3041939093 @default.
• W3195852110 cites W3107605961 @default.
• W3195852110 cites W3108604517 @default.
• W3195852110 cites W3118553819 @default.
• W3195852110 cites W3177725658 @default.
• W3195852110 cites W88272206 @default.
• W3195852110 doi "https://doi.org/10.1021/acsmeasuresciau.1c00007" @default.
• W3195852110 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8377714" @default.
• W3195852110 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34476422" @default.
• W3195852110 hasPublicationYear "2021" @default.
• W3195852110 type Work @default.
• W3195852110 sameAs 3195852110 @default.
• W3195852110 citedByCount "16" @default.
• W3195852110 countsByYear W31958521102021 @default.
• W3195852110 countsByYear W31958521102022 @default.
• W3195852110 countsByYear W31958521102023 @default.
• W3195852110 crossrefType "journal-article" @default.
• W3195852110 hasAuthorship W3195852110A5004347939 @default.
• W3195852110 hasAuthorship W3195852110A5006252438 @default.
• W3195852110 hasAuthorship W3195852110A5027513211 @default.
• W3195852110 hasAuthorship W3195852110A5044117588 @default.
• W3195852110 hasAuthorship W3195852110A5063652834 @default.
• W3195852110 hasAuthorship W3195852110A5085399017 @default.
• W3195852110 hasBestOaLocation W31958521103 @default.
• W3195852110 hasConcept C121332964 @default.
• W3195852110 hasConcept C162356407 @default.
• W3195852110 hasConcept C172493649 @default.
• W3195852110 hasConcept C185592680 @default.
• W3195852110 hasConcept C21565614 @default.
• W3195852110 hasConcept C2780841973 @default.
• W3195852110 hasConcept C31827203 @default.
• W3195852110 hasConcept C33390570 @default.
• W3195852110 hasConcept C43617362 @default.
• W3195852110 hasConcept C62520636 @default.
• W3195852110 hasConcept C71043704 @default.
• W3195852110 hasConceptScore W3195852110C121332964 @default.
• W3195852110 hasConceptScore W3195852110C162356407 @default.
• W3195852110 hasConceptScore W3195852110C172493649 @default.
• W3195852110 hasConceptScore W3195852110C185592680 @default.
• W3195852110 hasConceptScore W3195852110C21565614 @default.
• W3195852110 hasConceptScore W3195852110C2780841973 @default.
• W3195852110 hasConceptScore W3195852110C31827203 @default.
• W3195852110 hasConceptScore W3195852110C33390570 @default.
• W3195852110 hasConceptScore W3195852110C43617362 @default.
• W3195852110 hasConceptScore W3195852110C62520636 @default.
• W3195852110 hasConceptScore W3195852110C71043704 @default.
• W3195852110 hasFunder F4320332161 @default.
• W3195852110 hasIssue "1" @default.
• W3195852110 hasLocation W31958521101 @default.
• W3195852110 hasLocation W31958521102 @default.
• W3195852110 hasLocation W31958521103 @default.

• next