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- W3196310640 abstract "A cDNA fragment encoding a putative protein of elongat ion factor 1 alpha (EF1a) was ifrstly isolated from Cinnamomum camphora by using homology cloning, A pair of degenerate primers was designed based on the conserved sequences of other EF1a genes from the different species from GenBank, and a fragment of 1 297 bp was obtained through Reverse Transcription Polymerase Chain Reaction (RT-PCR) techniques. The results of homologous alignment show that CcEF1a shared over 80%nucleotide sequence identity and over 96%amino acid sequence identity with EF1a genes in other plants that had been submitted to GenBank. The cDNA fragment sequence was named CcEF1a and registered in GenBank with accession number KM086740. According to the results of real-time quantitative PCR, the mRNA level of CcEF1a in leaves under chilling stress was identical. It could be used as an interrnal control in real-time quantitative PCR." @default.
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- W3196310640 date "2015-01-01" @default.
- W3196310640 modified "2023-09-23" @default.
- W3196310640 title "Cloning and expression analysis of EF1a gene fragment of elongation factor from Cinnamomum camphora" @default.
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