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- W3196558143 abstract "Abstract Cell surface membrane proteins perform diverse and critical functions and are spatially and temporally regulated by membrane trafficking pathways. Although perturbations in these pathways underlie many pathologies, our understanding of these pathways at a mechanistic level remains incomplete. Using yeast as a model, we have developed an assay that reports on the surface activity of the uracil permease Fur4 in uracil auxotroph strains grown in the presence of limited uracil. This assay was used to screen a library of haploid deletion strains and identified mutants with both diminished and enhanced comparative growth in restricted uracil media. Factors identified, including various multisubunit complexes, were enriched for membrane trafficking and transcriptional functions, in addition to various uncharacterized genes. Bioinformatic analysis of expression profiles from many strains lacking transcription factors required for efficient uracil‐scavenging validated particular hits from the screen, in addition to implicating essential genes not tested in the screen. Finally, we performed a secondary mating factor secretion screen to functionally categorize factors implicated in uracil‐scavenging." @default.
- W3196558143 created "2021-09-13" @default.
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- W3196558143 date "2021-09-23" @default.
- W3196558143 modified "2023-09-24" @default.
- W3196558143 title "Fur4‐mediated uracil‐scavenging to screen for surface protein regulators" @default.
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- W3196558143 doi "https://doi.org/10.1111/tra.12815" @default.
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