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- W3196609249 abstract "ABSTRACT Conventional techniques for the extraction of the melanin encounter several disadvantages such as poor extraction efficiency, excessive use of acid and alkali, and lengthy time duration. To extract melanin in a more sustainable manner, herein suitability of an aqueous solution of quaternary ammonium hydroxide known as tetrabutylammonium hydroxide (40% w/w TBAOH in water) to extract and purify the pigment from the endophyte Streptomyces hyderabadensis 7VPT5-5R was investigated. The solvent could extract 5.54±0.03 g/L of pure melanin against 3.31± 0.02 g/L of pure melanin by conventional method from bacterial culture resulting 66% increase in the yield of the pigment. The quaternary ammonium based solvent was further recycled for 5 consecutive cycles and production of melanin with yield ranging from 5.54±0.03 g/L to 5.47± 0.02 g/L was obtained. The yield of the pigment was 38−40% higher in the recycled solvent in comparison to the melanin yield extracted by conventional method. Considering the efficiency of the solvent for effective extraction and purification of melanin and its recyclability potential, it can be considered as a sustainable solvent for the extraction of the pigments from bacterial culture. Furthermore, the developed method for extraction of melanin from microorganisms as a sustainable source would help in reducing the cost of melanin leading to its efficient utilization in diverse fields." @default.
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- W3196609249 date "2021-05-10" @default.
- W3196609249 modified "2023-10-10" @default.
- W3196609249 title "A Facile Alternative Sustainable Process for the Extraction and Purification of Microbial Melanin" @default.
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- W3196609249 doi "https://doi.org/10.1101/2021.05.10.443376" @default.
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