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- W3196622210 abstract "Dictyostelid social amoebae are a highly diverse group of eukaryotic soil microbes that are valuable resources for biological research. Genetic diversity study of these organisms solely relies on molecular phylogenetics of the SSU rDNA gene, which is not ideal for large-scale genetic diversity study. Here, we designed a set of PCR–single-strand conformation polymorphism (SSCP) primers and optimized the SSCP fingerprint method for the screening of dictyostelids. The optimized SSCP condition required gel purification of the SSCP amplicons followed by electrophoresis using a 9% polyacrylamide gel under 4°C. We also tested the optimized SSCP procedure with 73 Thai isolates of dictyostelid that had the SSU rDNA gene sequences published. The SSCP fingerprint patterns were related to the genus-level taxonomy of dictyostelids, but the fingerprint dendrogram did not reflect the deep phylogeny. This method is rapid, cost-effective, and suitable for large-scale sample screening as compared with the phylogenetic analysis of the SSU rDNA gene sequences." @default.
- W3196622210 created "2021-09-13" @default.
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- W3196622210 date "2021-08-27" @default.
- W3196622210 modified "2023-09-27" @default.
- W3196622210 title "Single-Strand Conformation Polymorphism Fingerprint Method for Dictyostelids" @default.
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- W3196622210 doi "https://doi.org/10.3389/fmicb.2021.708685" @default.
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