FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3196678128> ?p ?o ?g. }

• W3196678128 endingPage "100115" @default.
• W3196678128 startingPage "100115" @default.
• W3196678128 abstract "Lipases constitute an important class of water-soluble enzymes that catalyze the hydrolysis of hydrophobic triacylglycerol (TAG). Their enzymatic activity is typically measured using multistep procedures involving isolation and quantification of the hydrolyzed products. We report here a new fluorescence method to measure lipase activity in real time that does not require the separation of substrates from products. We developed this method using adipose triglyceride lipase (ATGL) and lipoprotein lipase (LpL) as model lipases. We first incubated a source of ATGL or LpL with substrate vesicles containing nitrobenzoxadiazole (NBD)-labeled TAG, then measured increases in NBD fluorescence, and calculated enzyme activities. Incorporation of NBD-TAG into phosphatidylcholine (PC) vesicles resulted in some hydrolysis; however, incorporation of phosphatidylinositol into these NBD-TAG/PC vesicles and increasing the ratio of NBD-TAG to PC greatly enhanced substrate hydrolysis. This assay was also useful in measuring the activity of pancreatic lipase and hormone-sensitive lipase. Next, we tested several small-molecule lipase inhibitors and found that orlistat inhibits all lipases, indicating that it is a pan-lipase inhibitor. In short, we describe a simple, rapid, fluorescence-based triacylglycerol hydrolysis assay to assess four major TAG hydrolases: intracellular ATGL and hormone-sensitive lipase, LpL localized at the extracellular endothelium, and pancreatic lipase present in the intestinal lumen. The major advantages of this method are its speed, simplicity, and elimination of product isolation. This assay is potentially applicable to a wide range of lipases, is amenable to high-throughput screening to discover novel modulators of triacylglycerol hydrolases, and can be used for diagnostic purposes." @default.
• W3196678128 created "2021-09-13" @default.
• W3196678128 creator A5009748611 @default.
• W3196678128 creator A5055404631 @default.
• W3196678128 creator A5057043472 @default.
• W3196678128 creator A5066507748 @default.
• W3196678128 creator A5086157288 @default.
• W3196678128 date "2021-01-01" @default.
• W3196678128 modified "2023-10-16" @default.
• W3196678128 title "A simple, rapid, and sensitive fluorescence-based method to assess triacylglycerol hydrolase activity" @default.
• W3196678128 cites W1511097185 @default.
• W3196678128 cites W1886303132 @default.
• W3196678128 cites W1975892680 @default.
• W3196678128 cites W1977847461 @default.
• W3196678128 cites W1991350073 @default.
• W3196678128 cites W1995092745 @default.
• W3196678128 cites W1999683222 @default.
• W3196678128 cites W2017650269 @default.
• W3196678128 cites W2023977281 @default.
• W3196678128 cites W2030843949 @default.
• W3196678128 cites W2031967041 @default.
• W3196678128 cites W2032496660 @default.
• W3196678128 cites W2039552793 @default.
• W3196678128 cites W2041719291 @default.
• W3196678128 cites W2044118255 @default.
• W3196678128 cites W2048994688 @default.
• W3196678128 cites W2052261620 @default.
• W3196678128 cites W2058668243 @default.
• W3196678128 cites W2062133242 @default.
• W3196678128 cites W2072129661 @default.
• W3196678128 cites W2072967808 @default.
• W3196678128 cites W2090847035 @default.
• W3196678128 cites W2090946848 @default.
• W3196678128 cites W2092233698 @default.
• W3196678128 cites W2095530389 @default.
• W3196678128 cites W2096596761 @default.
• W3196678128 cites W2104849027 @default.
• W3196678128 cites W2110839026 @default.
• W3196678128 cites W2112520822 @default.
• W3196678128 cites W2114525758 @default.
• W3196678128 cites W2114695967 @default.
• W3196678128 cites W2115731835 @default.
• W3196678128 cites W2129744834 @default.
• W3196678128 cites W2131507044 @default.
• W3196678128 cites W2134234726 @default.
• W3196678128 cites W2138267862 @default.
• W3196678128 cites W2142488399 @default.
• W3196678128 cites W2145642828 @default.
• W3196678128 cites W2148235740 @default.
• W3196678128 cites W2167914387 @default.
• W3196678128 cites W2170210469 @default.
• W3196678128 cites W2191503198 @default.
• W3196678128 cites W2215606085 @default.
• W3196678128 cites W2220000844 @default.
• W3196678128 cites W2253374107 @default.
• W3196678128 cites W2319953058 @default.
• W3196678128 cites W2327047826 @default.
• W3196678128 cites W2334579686 @default.
• W3196678128 cites W2343751886 @default.
• W3196678128 cites W2344077070 @default.
• W3196678128 cites W2406609013 @default.
• W3196678128 cites W2423918959 @default.
• W3196678128 cites W2428150038 @default.
• W3196678128 cites W2601642277 @default.
• W3196678128 cites W2618984243 @default.
• W3196678128 cites W2730976293 @default.
• W3196678128 cites W2750859969 @default.
• W3196678128 cites W2793043596 @default.
• W3196678128 cites W2947847131 @default.
• W3196678128 cites W2964430789 @default.
• W3196678128 cites W2998620436 @default.
• W3196678128 cites W3005056868 @default.
• W3196678128 cites W3019109939 @default.
• W3196678128 cites W3089208549 @default.
• W3196678128 cites W4230306741 @default.
• W3196678128 doi "https://doi.org/10.1016/j.jlr.2021.100115" @default.
• W3196678128 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8488599" @default.
• W3196678128 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34508728" @default.
• W3196678128 hasPublicationYear "2021" @default.
• W3196678128 type Work @default.
• W3196678128 sameAs 3196678128 @default.
• W3196678128 citedByCount "7" @default.
• W3196678128 countsByYear W31966781282022 @default.
• W3196678128 countsByYear W31966781282023 @default.
• W3196678128 crossrefType "journal-article" @default.
• W3196678128 hasAuthorship W3196678128A5009748611 @default.
• W3196678128 hasAuthorship W3196678128A5055404631 @default.
• W3196678128 hasAuthorship W3196678128A5057043472 @default.
• W3196678128 hasAuthorship W3196678128A5066507748 @default.
• W3196678128 hasAuthorship W3196678128A5086157288 @default.
• W3196678128 hasBestOaLocation W31966781281 @default.
• W3196678128 hasConcept C130316041 @default.
• W3196678128 hasConcept C170493617 @default.
• W3196678128 hasConcept C181199279 @default.
• W3196678128 hasConcept C185592680 @default.
• W3196678128 hasConcept C18903297 @default.
• W3196678128 hasConcept C2777062983 @default.
• W3196678128 hasConcept C2777289219 @default.

• next