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- W3196785207 abstract "ABSTRAK : Monospecific abortus serum and monospecific melitensis serum have been prepared in rabbits. The aim of this applied research was to obtain suitable methods for preparation of standard biological reagents for the diagnosis of brucellosis and biotyping of brucella isolates from livestock in Indonesia. The results of this work will be of considerable importance in the epidemiological studies of brucellosis in Indonesia. Reference strain of Brucella abortus (S.19 and S.544) and Brucella melitensis (626) were used and the rabbits used were New Zeland White cross breed. Suspension of brucella organisms (killed and living) were used as antigens to produce antisera in the rabbits. After exsanguinating those animals with high agglutinin titres (1 is greater than or equal to divided by 320), separate pools of antisera against B. abortus and B. melitensis were made. Packed brucella cells of the heterologous species were used to absorb each batch of serum by mixing and incubating in a water bath at 37 degrees Celcius for two hours to remove the cross-reacting agglutinins. After absorption, the brucella cells were removed by centrifugation and the absorbed serum tested by slide agglutination with homologus and heterologous brucella suspension. By these methods several batches of monospecific B.abortus and B.melitensis antisera were produced which gave satisfatory reactions." @default.
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- W3196785207 date "1985-01-01" @default.
- W3196785207 modified "2023-09-27" @default.
- W3196785207 title "Production of monospecific antisera for Brucella abortus and B.melitensis = Pembuatan antisera monospesifik Brucella abortus dan Brucella melitensis penelitian pendahuluan" @default.
- W3196785207 hasPublicationYear "1985" @default.
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