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- W3196950599 abstract "Fast solution-mediated solid-state conversion of d-threonine (d-Thr) to l-allothreonine (l-aThr) was achieved using temperature cycles and enzymatic epimerization catalyzed by an immobilized amino acid racemase (imAAR). The conversion is due to the higher stability of the l-aThr crystals compared to the d-Thr crystals and the ability of the molecules to interconvert in the liquid phase. Applying temperature cycles greatly accelerated the conversion of Thr to aThr. Furthermore, the mechanism of the solid-state conversion was demonstrated via the progression of diastereomeric excess in the solid and liquid phases, the transmissivity of the suspension, and in situ images. The rate of the process can be altered via adjustment of the temperature difference used in the cycle, the cooling rate, and the dosage of imAAR. The process is reproducible and characterized by reasonable values for the productivity and yield." @default.
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- W3196950599 date "2021-08-26" @default.
- W3196950599 modified "2023-09-23" @default.
- W3196950599 title "Efficient Conversion of Threonine to Allothreonine Using Immobilized Amino Acid Racemase and Temperature Cycles" @default.
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- W3196950599 doi "https://doi.org/10.1021/acs.cgd.1c00484" @default.
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