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• W3197027706 endingPage "e0257168" @default.
• W3197027706 startingPage "e0257168" @default.
• W3197027706 abstract "Shiga toxin-producing Escherichia coli (STEC) have been linked to food-borne disease outbreaks. As PCR is routinely used to screen foods for STEC, it is important that factors leading to inconsistent detection of STEC by PCR are understood. This study used whole genome sequencing (WGS) to investigate causes of inconsistent PCR detection of stx 1 , stx 2 , and serogroup-specific genes. Fifty strains isolated from Alberta feedlot cattle from three different studies were selected with inconsistent or consistent detection of stx and serogroup by PCR. All isolates were initially classified as STEC by PCR. Sequencing was performed using Illumina MiSeq® with sample library by Nextera XT. Virtual PCRs were performed using Geneious and bacteriophage content was determined using PHASTER. Sequencing coverage ranged from 47 to 102x, averaging 74x, with sequences deposited in the NCBI database. Eleven strains were confirmed by WGS as STEC having complete stxA and stxB subunits. However, truncated stx fragments occurred in twenty-two other isolates, some having multiple stx fragments in the genome. Isolates with complete stx by WGS had consistent stx 1 and stx 2 detection by PCR, although one also having a stx 2 fragment had inconsistent stx 2 PCR. For all STEC and 18/39 non-STEC, serogroups determined by PCR agreed with those determined by WGS. An additional three WGS serotypes were inconclusive and two isolates were Citrobacter spp. Results demonstrate that stx fragments associated with stx -carrying bacteriophages in the E . coli genome may contribute to inconsistent detection of stx 1 and stx 2 by PCR. Fourteen isolates had integrated stx bacteriophage but lacked complete or fragmentary stx possibly due to partial bacteriophage excision after sub-cultivation or other unclear mechanisms. The majority of STEC isolates (7/11) did not have identifiable bacteriophage DNA in the contig(s) where stx was located, likely increasing the stability of stx in the bacterial genome and its detection by PCR." @default.
• W3197027706 created "2021-09-13" @default.
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• W3197027706 date "2021-09-03" @default.
• W3197027706 modified "2023-10-01" @default.
• W3197027706 title "Inconsistent PCR detection of Shiga toxin-producing Escherichia coli: Insights from whole genome sequence analyses" @default.
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• W3197027706 doi "https://doi.org/10.1371/journal.pone.0257168" @default.
• W3197027706 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8415614" @default.
• W3197027706 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34478476" @default.
• W3197027706 hasPublicationYear "2021" @default.
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