FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3197054377> ?p ?o ?g. }

• W3197054377 abstract "BACKGROUND Trypanosoma cruzi is an important human pathogen in Latin America with nearly seven million people infected. It has a large degree of genetic diversity, classified into six discrete typing units (DTUs), which probably influences its physiological behavior and clinical manifestations. Several genotyping methods are available, with distinct performance on easiness, cost, resolution and applicability; no method excels in all parameters. OBJECTIVES AND METHODS To devise a molecular method for T. cruzi genotyping, based on polymerase chain reaction (PCR) amplification of a single target with multiple copies in the nuclear genome by large scale sequencing. We have applied this method to 29 T. cruzi isolates, comprising all described DTUs. FINDINGS We were able to classify all samples into sub DTU level with high robustness. Evolutionary relationship between DTUs were ascertained, suggesting that TcIII and TcIV DTUs are non-hybrid, and DTU IV is more similar to the common ancestral. CONCLUSION As the TS-LSS method is based on a single PCR reaction, comprising several copies of the target, it is probably useful for clinical samples, when the amount of DNA is a limiting factor. As large scale sequencing systems become more common, the TS-LSS method can be increasingly applied for T. cruzi genotyping." @default.
• W3197054377 created "2021-09-13" @default.
• W3197054377 creator A5014937327 @default.
• W3197054377 creator A5018348740 @default.
• W3197054377 creator A5034252481 @default.
• W3197054377 creator A5041422655 @default.
• W3197054377 creator A5049746814 @default.
• W3197054377 creator A5078377372 @default.
• W3197054377 creator A5088622218 @default.
• W3197054377 creator A5090579066 @default.
• W3197054377 date "2021-01-01" @default.
• W3197054377 modified "2023-10-02" @default.
• W3197054377 title "A new Trypanosoma cruzi genotyping method enables high resolution evolutionary analyses" @default.
• W3197054377 cites W1487790589 @default.
• W3197054377 cites W1712511248 @default.
• W3197054377 cites W1920782262 @default.
• W3197054377 cites W1970788359 @default.
• W3197054377 cites W1979692840 @default.
• W3197054377 cites W1988929438 @default.
• W3197054377 cites W1998306753 @default.
• W3197054377 cites W2021858407 @default.
• W3197054377 cites W2031971139 @default.
• W3197054377 cites W2037147698 @default.
• W3197054377 cites W2049102964 @default.
• W3197054377 cites W2059981817 @default.
• W3197054377 cites W2061246349 @default.
• W3197054377 cites W2063382384 @default.
• W3197054377 cites W2074930676 @default.
• W3197054377 cites W2098634984 @default.
• W3197054377 cites W2109174028 @default.
• W3197054377 cites W2112479590 @default.
• W3197054377 cites W2113946934 @default.
• W3197054377 cites W2119284644 @default.
• W3197054377 cites W2124166542 @default.
• W3197054377 cites W2124715997 @default.
• W3197054377 cites W2127315472 @default.
• W3197054377 cites W2150550043 @default.
• W3197054377 cites W2150774511 @default.
• W3197054377 cites W2151551515 @default.
• W3197054377 cites W2153164685 @default.
• W3197054377 cites W2154959095 @default.
• W3197054377 cites W2155159495 @default.
• W3197054377 cites W2157701114 @default.
• W3197054377 cites W2159440462 @default.
• W3197054377 cites W2169307994 @default.
• W3197054377 cites W2182268609 @default.
• W3197054377 cites W2285967316 @default.
• W3197054377 cites W2336330109 @default.
• W3197054377 cites W244233237 @default.
• W3197054377 cites W2586995824 @default.
• W3197054377 cites W2609677605 @default.
• W3197054377 cites W2612301982 @default.
• W3197054377 cites W4210980497 @default.
• W3197054377 doi "https://doi.org/10.1590/0074-02760200538" @default.
• W3197054377 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8405150" @default.
• W3197054377 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34468503" @default.
• W3197054377 hasPublicationYear "2021" @default.
• W3197054377 type Work @default.
• W3197054377 sameAs 3197054377 @default.
• W3197054377 citedByCount "3" @default.
• W3197054377 countsByYear W31970543772022 @default.
• W3197054377 countsByYear W31970543772023 @default.
• W3197054377 crossrefType "journal-article" @default.
• W3197054377 hasAuthorship W3197054377A5014937327 @default.
• W3197054377 hasAuthorship W3197054377A5018348740 @default.
• W3197054377 hasAuthorship W3197054377A5034252481 @default.
• W3197054377 hasAuthorship W3197054377A5041422655 @default.
• W3197054377 hasAuthorship W3197054377A5049746814 @default.
• W3197054377 hasAuthorship W3197054377A5078377372 @default.
• W3197054377 hasAuthorship W3197054377A5088622218 @default.
• W3197054377 hasAuthorship W3197054377A5090579066 @default.
• W3197054377 hasBestOaLocation W31970543771 @default.
• W3197054377 hasConcept C104317684 @default.
• W3197054377 hasConcept C135763542 @default.
• W3197054377 hasConcept C136764020 @default.
• W3197054377 hasConcept C141231307 @default.
• W3197054377 hasConcept C2777625466 @default.
• W3197054377 hasConcept C2781209916 @default.
• W3197054377 hasConcept C31467283 @default.
• W3197054377 hasConcept C41008148 @default.
• W3197054377 hasConcept C49105822 @default.
• W3197054377 hasConcept C51679486 @default.
• W3197054377 hasConcept C54355233 @default.
• W3197054377 hasConcept C552990157 @default.
• W3197054377 hasConcept C70721500 @default.
• W3197054377 hasConcept C71928629 @default.
• W3197054377 hasConcept C86803240 @default.
• W3197054377 hasConceptScore W3197054377C104317684 @default.
• W3197054377 hasConceptScore W3197054377C135763542 @default.
• W3197054377 hasConceptScore W3197054377C136764020 @default.
• W3197054377 hasConceptScore W3197054377C141231307 @default.
• W3197054377 hasConceptScore W3197054377C2777625466 @default.
• W3197054377 hasConceptScore W3197054377C2781209916 @default.
• W3197054377 hasConceptScore W3197054377C31467283 @default.
• W3197054377 hasConceptScore W3197054377C41008148 @default.
• W3197054377 hasConceptScore W3197054377C49105822 @default.
• W3197054377 hasConceptScore W3197054377C51679486 @default.
• W3197054377 hasConceptScore W3197054377C54355233 @default.
• W3197054377 hasConceptScore W3197054377C552990157 @default.
• W3197054377 hasConceptScore W3197054377C70721500 @default.

• next