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- W3197104532 abstract "Sensitivity is often an issue in bioanalytical LC-MS/MS applications. Commonly investigated parameters to improve it include additives to mobile phase, derivatization and sample-preparation. The nature of the column, however, is not frequently evaluated. The sensitivity is compared for 18 different reversed phase and 2 different HILIC columns using 2 different mobile phase compositions. Sensitivity was evaluated in terms of S/N for 1,5 pg oxytocin on column, using a scouting gradient. The measured signal to noise ranged from 55 to 1473, indicating a substantial difference in sensitivity. The most sensitive columns were the Synergi Hydro RP (reversed phase) and the Atlantis HILIC (HILIC). This study shows that choosing the right column contributes to the sensitivity of the method." @default.
- W3197104532 created "2021-09-13" @default.
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- W3197104532 date "2021-09-01" @default.
- W3197104532 modified "2023-09-23" @default.
- W3197104532 title "Optimizing oxytocin LC-MS/MS sensitivity by choosing the right column" @default.
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- W3197104532 doi "https://doi.org/10.1016/j.plabm.2021.e00254" @default.
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