Matches in SemOpenAlex for { <https://semopenalex.org/work/W3197181812> ?p ?o ?g. }
- W3197181812 abstract "Third-generation sequencing has penetrated little in metagenomics due to the high error rate and dependence for assembly on short-read designed bioinformatics. However, second-generation sequencing metagenomics (mostly Illumina) suffers from limitations, particularly in the assembly of microbes with high microdiversity and retrieval of the flexible (adaptive) fraction of prokaryotic genomes. Here, we have used a third-generation technique to study the metagenome of a well-known marine sample from the mixed epipelagic water column of the winter Mediterranean. We have compared PacBio Sequel II with the classical approach using Illumina Nextseq short reads followed by assembly to study the metagenome. Long reads allow for efficient direct retrieval of complete genes avoiding the bias of the assembly step. Besides, the application of long reads on metagenomic assembly allows for the reconstruction of much more complete metagenome-assembled genomes (MAGs), particularly from microbes with high microdiversity such as Pelagibacterales. The flexible genome of reconstructed MAGs was much more complete containing many adaptive genes (some with biotechnological potential). PacBio Sequel II CCS appears particularly suitable for cellular metagenomics due to its low error rate. For most applications of metagenomics, from community structure analysis to ecosystem functioning, long reads should be applied whenever possible. Specifically, for in silico screening of biotechnologically useful genes, or population genomics, long-read metagenomics appears presently as a very fruitful approach and can be analyzed from raw reads before a computationally demanding (and potentially artifactual) assembly step." @default.
- W3197181812 created "2021-09-13" @default.
- W3197181812 creator A5046713841 @default.
- W3197181812 creator A5055895482 @default.
- W3197181812 creator A5084961018 @default.
- W3197181812 date "2021-08-27" @default.
- W3197181812 modified "2023-10-18" @default.
- W3197181812 title "Enhanced Recovery of Microbial Genes and Genomes From a Marine Water Column Using Long-Read Metagenomics" @default.
- W3197181812 cites W1519765314 @default.
- W3197181812 cites W1861776684 @default.
- W3197181812 cites W1939004772 @default.
- W3197181812 cites W1956297315 @default.
- W3197181812 cites W1977739164 @default.
- W3197181812 cites W1980298106 @default.
- W3197181812 cites W1985048665 @default.
- W3197181812 cites W1990118801 @default.
- W3197181812 cites W1991604124 @default.
- W3197181812 cites W1996423252 @default.
- W3197181812 cites W2004068562 @default.
- W3197181812 cites W2005102671 @default.
- W3197181812 cites W2007211860 @default.
- W3197181812 cites W2008264573 @default.
- W3197181812 cites W2010312902 @default.
- W3197181812 cites W2024752819 @default.
- W3197181812 cites W2026315105 @default.
- W3197181812 cites W2030569406 @default.
- W3197181812 cites W2034285706 @default.
- W3197181812 cites W2037015298 @default.
- W3197181812 cites W2037084529 @default.
- W3197181812 cites W2039352303 @default.
- W3197181812 cites W2045204781 @default.
- W3197181812 cites W2047032063 @default.
- W3197181812 cites W2075592322 @default.
- W3197181812 cites W2079075989 @default.
- W3197181812 cites W2083595411 @default.
- W3197181812 cites W2093830129 @default.
- W3197181812 cites W2095498620 @default.
- W3197181812 cites W2097606916 @default.
- W3197181812 cites W2103539617 @default.
- W3197181812 cites W2105905824 @default.
- W3197181812 cites W2108058966 @default.
- W3197181812 cites W2109867978 @default.
- W3197181812 cites W2111647009 @default.
- W3197181812 cites W2112364185 @default.
- W3197181812 cites W2113601822 @default.
- W3197181812 cites W2114200698 @default.
- W3197181812 cites W2114392707 @default.
- W3197181812 cites W2117939087 @default.
- W3197181812 cites W2119888547 @default.
- W3197181812 cites W2119923823 @default.
- W3197181812 cites W2120902911 @default.
- W3197181812 cites W2122559203 @default.
- W3197181812 cites W2124351063 @default.
- W3197181812 cites W2126285646 @default.
- W3197181812 cites W2127175247 @default.
- W3197181812 cites W2131271579 @default.
- W3197181812 cites W2132341951 @default.
- W3197181812 cites W2132926880 @default.
- W3197181812 cites W2134800079 @default.
- W3197181812 cites W2138122982 @default.
- W3197181812 cites W2138730482 @default.
- W3197181812 cites W2141920662 @default.
- W3197181812 cites W2143485490 @default.
- W3197181812 cites W2146079368 @default.
- W3197181812 cites W2146384743 @default.
- W3197181812 cites W2149429041 @default.
- W3197181812 cites W2158714788 @default.
- W3197181812 cites W2159954944 @default.
- W3197181812 cites W2166123725 @default.
- W3197181812 cites W2168749192 @default.
- W3197181812 cites W2169929748 @default.
- W3197181812 cites W2171777099 @default.
- W3197181812 cites W2179507357 @default.
- W3197181812 cites W2191058900 @default.
- W3197181812 cites W2195724570 @default.
- W3197181812 cites W2283279263 @default.
- W3197181812 cites W2303521084 @default.
- W3197181812 cites W2335786001 @default.
- W3197181812 cites W2342192228 @default.
- W3197181812 cites W2344821303 @default.
- W3197181812 cites W2345730070 @default.
- W3197181812 cites W2396849069 @default.
- W3197181812 cites W2399809721 @default.
- W3197181812 cites W2465681871 @default.
- W3197181812 cites W2473175534 @default.
- W3197181812 cites W2495141184 @default.
- W3197181812 cites W2520785555 @default.
- W3197181812 cites W2525132236 @default.
- W3197181812 cites W2559574135 @default.
- W3197181812 cites W2563361853 @default.
- W3197181812 cites W2575929740 @default.
- W3197181812 cites W2605920051 @default.
- W3197181812 cites W2607669908 @default.
- W3197181812 cites W2745006471 @default.
- W3197181812 cites W2753961085 @default.
- W3197181812 cites W2759689312 @default.
- W3197181812 cites W2763159727 @default.
- W3197181812 cites W2766750334 @default.
- W3197181812 cites W2792743692 @default.
- W3197181812 cites W2803245377 @default.