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- W3197247995 endingPage "3323" @default.
- W3197247995 startingPage "3304" @default.
- W3197247995 abstract "Post-translational modifications frequently modulate protein functions. Lysine acetylation in particular plays a key role in interactions between respiratory cytochrome c and its metabolic partners. To date, in vivo acetylation of lysines at positions 8 and 53 has specifically been identified in mammalian cytochrome c, but little is known about the structural basis of acetylation-induced functional changes. Here, we independently replaced these two residues in recombinant human cytochrome c with glutamine to mimic lysine acetylation and then characterized the structure and function of the resulting K8Q and K53Q mutants. We found that the physicochemical features were mostly unchanged in the two acetyl-mimetic mutants, but their thermal stability was significantly altered. NMR chemical shift perturbations of the backbone amide resonances revealed local structural changes, and the thermodynamics and kinetics of electron transfer in mutants immobilized on gold electrodes showed an increase in both protein dynamics and solvent involvement in the redox process. We also observed that the K8Q (but not the K53Q) mutation slightly increased the binding affinity of cytochrome c to its physiological electron donor, cytochrome c1 -which is a component of mitochondrial complex III, or cytochrome bc1 -thus suggesting that Lys8 (but not Lys53) is located in the interaction area. Finally, the K8Q and K53Q mutants exhibited reduced efficiency as electron donors to complex IV, or cytochrome c oxidase." @default.
- W3197247995 created "2021-09-13" @default.
- W3197247995 creator A5023014264 @default.
- W3197247995 creator A5033076593 @default.
- W3197247995 creator A5049125752 @default.
- W3197247995 creator A5051551027 @default.
- W3197247995 creator A5067563831 @default.
- W3197247995 creator A5074740504 @default.
- W3197247995 creator A5076363284 @default.
- W3197247995 creator A5086895428 @default.
- W3197247995 date "2021-11-09" @default.
- W3197247995 modified "2023-10-18" @default.
- W3197247995 title "Structural and functional insights into lysine acetylation of cytochrome c using mimetic point mutants" @default.
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