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- W3197346737 abstract "We describe the design of peptides with properties like thermostability, pH stability, and antibacterial activity against a few bacterial food pathogens. Insights obtained from classical structure-function analysis of natural peptides and their mutants through antimicrobial and enzymatic assays are used to rationally develop a set of peptides. pH and thermostability assays were performed to demonstrate robust antimicrobial activity post-treatment with high temperatures and at wide pH ranges. We have also investigated the mode of action of these hyperstable peptides using membrane permeability assays, electron microscopy, and molecular dynamics simulations. Notably, through mutational studies, we show that these peptides elicit their antibacterial action via both membrane destabilization and inhibition of intracellular trypsin-the two functions attributable to separate peptide segments. Finally, toxicity studies and food preservation assays demonstrate the safety and efficacy of the designed peptides for food preservation. Overall, the study provides a general 'blueprint' for the development of stable antimicrobial peptides (AMPs). Insights obtained from this work may also be combined with combinatorial methods in high-throughput studies for future development of antimicrobials for various applications." @default.
- W3197346737 created "2021-09-13" @default.
- W3197346737 creator A5037036671 @default.
- W3197346737 creator A5052000306 @default.
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- W3197346737 date "2021-09-01" @default.
- W3197346737 modified "2023-10-17" @default.
- W3197346737 title "Rational design of hyperstable antibacterial peptides for food preservation" @default.
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- W3197346737 cites W1917515870 @default.
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- W3197346737 cites W1973742248 @default.
- W3197346737 cites W1974980316 @default.
- W3197346737 cites W1981508312 @default.
- W3197346737 cites W1983489663 @default.
- W3197346737 cites W1984679573 @default.
- W3197346737 cites W1985284045 @default.
- W3197346737 cites W1985816651 @default.
- W3197346737 cites W1986873325 @default.
- W3197346737 cites W1989998611 @default.
- W3197346737 cites W1991794210 @default.
- W3197346737 cites W2006503674 @default.
- W3197346737 cites W2012417928 @default.
- W3197346737 cites W2013314308 @default.
- W3197346737 cites W2014922593 @default.
- W3197346737 cites W2015166285 @default.
- W3197346737 cites W2016198726 @default.
- W3197346737 cites W2021520922 @default.
- W3197346737 cites W2024650759 @default.
- W3197346737 cites W2025522718 @default.
- W3197346737 cites W2026145786 @default.
- W3197346737 cites W2029667189 @default.
- W3197346737 cites W2035266068 @default.
- W3197346737 cites W2035687084 @default.
- W3197346737 cites W2040125827 @default.
- W3197346737 cites W2041638382 @default.
- W3197346737 cites W2044077839 @default.
- W3197346737 cites W2054148289 @default.
- W3197346737 cites W2054979333 @default.
- W3197346737 cites W2055998978 @default.
- W3197346737 cites W2057477511 @default.
- W3197346737 cites W2059013803 @default.
- W3197346737 cites W2065283382 @default.
- W3197346737 cites W2067174909 @default.
- W3197346737 cites W2081693079 @default.
- W3197346737 cites W2083857695 @default.
- W3197346737 cites W2086382964 @default.
- W3197346737 cites W2086634221 @default.
- W3197346737 cites W2094942879 @default.
- W3197346737 cites W2107572441 @default.
- W3197346737 cites W2109154308 @default.
- W3197346737 cites W2132629607 @default.
- W3197346737 cites W2132651708 @default.
- W3197346737 cites W2133603290 @default.
- W3197346737 cites W2135964866 @default.
- W3197346737 cites W2136987348 @default.
- W3197346737 cites W2138447484 @default.
- W3197346737 cites W2141854312 @default.
- W3197346737 cites W2150152340 @default.
- W3197346737 cites W2163338412 @default.
- W3197346737 cites W2165779834 @default.
- W3197346737 cites W2167101814 @default.
- W3197346737 cites W2168812241 @default.
- W3197346737 cites W2169380117 @default.
- W3197346737 cites W2171727742 @default.
- W3197346737 cites W219291073 @default.
- W3197346737 cites W2201202899 @default.
- W3197346737 cites W2279347084 @default.
- W3197346737 cites W2324181628 @default.
- W3197346737 cites W2325581009 @default.
- W3197346737 cites W2507728244 @default.
- W3197346737 cites W2511368942 @default.
- W3197346737 cites W2536375018 @default.
- W3197346737 cites W2554425460 @default.
- W3197346737 cites W2555538399 @default.
- W3197346737 cites W2560792979 @default.
- W3197346737 cites W2562261325 @default.
- W3197346737 cites W2586156886 @default.
- W3197346737 cites W2617790881 @default.
- W3197346737 cites W2774210014 @default.
- W3197346737 cites W2902879129 @default.
- W3197346737 cites W2919451909 @default.
- W3197346737 cites W2920871075 @default.
- W3197346737 cites W2925117876 @default.
- W3197346737 cites W2948261638 @default.
- W3197346737 cites W2949753733 @default.
- W3197346737 cites W2999569426 @default.
- W3197346737 cites W3023583617 @default.
- W3197346737 cites W3048752454 @default.
- W3197346737 cites W4210968711 @default.
- W3197346737 cites W2092447988 @default.
- W3197346737 doi "https://doi.org/10.1038/s41538-021-00109-z" @default.
- W3197346737 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8410836" @default.
- W3197346737 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34471114" @default.
- W3197346737 hasPublicationYear "2021" @default.
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