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- W3197355545 abstract "Genetically transformed Bifidobacterium longum , a probiotic bacterium in the human intestines, has a high potential for in-situ delivery of therapeutic proteins. However, one limitation is its low transformation efficiency caused by its thick membrane as it is a Gram-positive bacterium. Here we present an excellent transformation efficiency of B. longum (up to 1 × 10 7 to 2.1 × 10 7 cells/μg of DNA), obtained when oxidized multi-walled carbon nanotubes are used in synergy with classic transformation methods. This approach opens a fruitful avenue for B. longum transformations that could increase its potential for in-situ delivery systems of recombinant proteins ." @default.
- W3197355545 created "2021-09-13" @default.
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- W3197355545 date "2021-10-01" @default.
- W3197355545 modified "2023-10-16" @default.
- W3197355545 title "Multi-walled carbon nanotubes enhance the genetic transformation of Bifidobacterium longum" @default.
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- W3197355545 doi "https://doi.org/10.1016/j.carbon.2021.08.052" @default.
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