FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3197605439> ?p ?o ?g. }

• W3197605439 endingPage "1131" @default.
• W3197605439 startingPage "1131" @default.
• W3197605439 abstract "As qualified microscopy of enteric parasitoses as defined by high diagnostic accuracy is difficult to maintain in non-endemic areas due to scarce opportunities for practicing with positive sample materials, molecular diagnostic options provide less investigator-dependent alternatives. Here, we compared three molecular targets for the real-time PCR-based detection of Cryptosporidium spp. From a population of 1000 individuals comprising both Ghanaian HIV (human immunodeficiency virus) patients and military returnees after deployment in the tropics, stool samples were assessed for Cryptosporidium spp. by real-time PCR targeting the small subunit ribosomal RNA (SSU rRNA) gene, the Cryptosporidium oocyst wall (COWP) gene, and the DnaJ-like protein gene (DnaJ), respectively. In declining order, sensitivity of 100% for the SSU rRNA gene PCR, 90.0% for the COWP PCR and 88.8% for the DnaJ PCR, respectively, as well as specificity of 99.6% for the COWP PCR and 96.9% for both the SSU rRNA gene PCR and the DnaJ PCR, respectively, were recorded. Substantial agreement (kappa value 0.663) between the three assays was observed. Further, an accuracy-adjusted Cryptosporidium spp. prevalence of 6.0% was calculated for the study population. In conclusion, none of the assessed real-time PCR assays were associated with perfect test accuracy. However, a combination of highly sensitive SSU rRNA gene PCR for screening purposes and more specific COWP PCR for confirmatory testing should allow reliable diagnosis of Cryptosporidium spp. in stool samples even in low prevalence settings." @default.
• W3197605439 created "2021-09-13" @default.
• W3197605439 creator A5013126710 @default.
• W3197605439 creator A5017961801 @default.
• W3197605439 creator A5051715028 @default.
• W3197605439 creator A5066201886 @default.
• W3197605439 creator A5068444829 @default.
• W3197605439 creator A5075537673 @default.
• W3197605439 creator A5076703292 @default.
• W3197605439 creator A5090810460 @default.
• W3197605439 date "2021-09-02" @default.
• W3197605439 modified "2023-10-10" @default.
• W3197605439 title "Comparison of Three Real-Time PCR Assays Targeting the SSU rRNA Gene, the COWP Gene and the DnaJ-Like Protein Gene for the Diagnosis of Cryptosporidium spp. in Stool Samples" @default.
• W3197605439 cites W1485932791 @default.
• W3197605439 cites W1667833068 @default.
• W3197605439 cites W1710994204 @default.
• W3197605439 cites W1838648354 @default.
• W3197605439 cites W1977544599 @default.
• W3197605439 cites W1979576376 @default.
• W3197605439 cites W1981463589 @default.
• W3197605439 cites W1982476458 @default.
• W3197605439 cites W1993092899 @default.
• W3197605439 cites W1994392659 @default.
• W3197605439 cites W2002503532 @default.
• W3197605439 cites W2005516777 @default.
• W3197605439 cites W2010814088 @default.
• W3197605439 cites W2015023236 @default.
• W3197605439 cites W2020872973 @default.
• W3197605439 cites W2030743386 @default.
• W3197605439 cites W2031454874 @default.
• W3197605439 cites W2032863095 @default.
• W3197605439 cites W2037013112 @default.
• W3197605439 cites W2040536528 @default.
• W3197605439 cites W2042504288 @default.
• W3197605439 cites W2055179720 @default.
• W3197605439 cites W2055867890 @default.
• W3197605439 cites W2058819939 @default.
• W3197605439 cites W2063518719 @default.
• W3197605439 cites W2071963226 @default.
• W3197605439 cites W2079093853 @default.
• W3197605439 cites W2085992834 @default.
• W3197605439 cites W2093352124 @default.
• W3197605439 cites W2103381558 @default.
• W3197605439 cites W2112717115 @default.
• W3197605439 cites W2113235881 @default.
• W3197605439 cites W2115573931 @default.
• W3197605439 cites W2116173667 @default.
• W3197605439 cites W2120625187 @default.
• W3197605439 cites W2121147515 @default.
• W3197605439 cites W2123559199 @default.
• W3197605439 cites W2123823729 @default.
• W3197605439 cites W2125693127 @default.
• W3197605439 cites W2131444418 @default.
• W3197605439 cites W2132433834 @default.
• W3197605439 cites W2134343377 @default.
• W3197605439 cites W2136002800 @default.
• W3197605439 cites W2139800456 @default.
• W3197605439 cites W2142662767 @default.
• W3197605439 cites W2143365807 @default.
• W3197605439 cites W2151389642 @default.
• W3197605439 cites W2156558869 @default.
• W3197605439 cites W2157550778 @default.
• W3197605439 cites W2157683553 @default.
• W3197605439 cites W2162649979 @default.
• W3197605439 cites W2164777277 @default.
• W3197605439 cites W2166504608 @default.
• W3197605439 cites W2170940707 @default.
• W3197605439 cites W2172780794 @default.
• W3197605439 cites W2198345065 @default.
• W3197605439 cites W2274657484 @default.
• W3197605439 cites W2284474987 @default.
• W3197605439 cites W2298570947 @default.
• W3197605439 cites W2321327455 @default.
• W3197605439 cites W2339501645 @default.
• W3197605439 cites W2501499001 @default.
• W3197605439 cites W2517721391 @default.
• W3197605439 cites W2528519179 @default.
• W3197605439 cites W2553311051 @default.
• W3197605439 cites W2563001009 @default.
• W3197605439 cites W2565736385 @default.
• W3197605439 cites W2608295290 @default.
• W3197605439 cites W2727435403 @default.
• W3197605439 cites W2751920778 @default.
• W3197605439 cites W2779546967 @default.
• W3197605439 cites W2791866425 @default.
• W3197605439 cites W2794017016 @default.
• W3197605439 cites W2893167551 @default.
• W3197605439 cites W2899224442 @default.
• W3197605439 cites W2899558278 @default.
• W3197605439 cites W2939029885 @default.
• W3197605439 cites W2939664793 @default.
• W3197605439 cites W2944997183 @default.
• W3197605439 cites W2949695952 @default.
• W3197605439 cites W2953326470 @default.
• W3197605439 cites W2970963809 @default.
• W3197605439 cites W2981370306 @default.
• W3197605439 cites W2995914391 @default.
• W3197605439 cites W3004137072 @default.

• next