FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3197894855> ?p ?o ?g. }

• W3197894855 endingPage "101186" @default.
• W3197894855 startingPage "101186" @default.
• W3197894855 abstract "Reactive oxygen species are key factors that strongly affect the cellular redox state and regulate various physiological and cellular phenomena. To monitor changes in the redox state, we previously developed fluorescent redox sensors named Re-Q, the emissions of which are quenched under reduced conditions. However, such fluorescent probes are unsuitable for use in the cells of photosynthetic organisms because they require photoexcitation that may change intracellular conditions and induce autofluorescence, primarily in chlorophylls. In addition, the presence of various chromophore pigments may interfere with fluorescence-based measurements because of their strong absorbance. To overcome these problems, we adopted the bioluminescence resonance energy transfer (BRET) mechanism for the sensor and developed two BRET-based redox sensors by fusing cyan fluorescent protein-based or yellow fluorescent protein-based Re-Q with the luminescent protein Nluc. We named the resulting redox-sensitive BRET-based indicator probes ROBINc and ROBINy. ROBINc is pH insensitive, which is especially vital for observation in photosynthetic organisms. By using these sensors, we successfully observed dynamic redox changes caused by an anticancer agent in HeLa cells and light/dark-dependent redox changes in the cells of photosynthetic cyanobacterium Synechocystis sp. PCC 6803. Since the newly developed sensors do not require excitation light, they should be especially useful for visualizing intracellular phenomena caused by redox changes in cells containing colored pigments." @default.
• W3197894855 created "2021-09-13" @default.
• W3197894855 creator A5001152422 @default.
• W3197894855 creator A5021865068 @default.
• W3197894855 creator A5040684273 @default.
• W3197894855 creator A5049221447 @default.
• W3197894855 creator A5055601896 @default.
• W3197894855 creator A5081980355 @default.
• W3197894855 date "2021-09-01" @default.
• W3197894855 modified "2023-09-28" @default.
• W3197894855 title "Monitoring cellular redox dynamics using newly developed BRET-based redox sensor proteins" @default.
• W3197894855 cites W1486131515 @default.
• W3197894855 cites W1894322524 @default.
• W3197894855 cites W1968071144 @default.
• W3197894855 cites W1968252310 @default.
• W3197894855 cites W1968294943 @default.
• W3197894855 cites W1970877840 @default.
• W3197894855 cites W1971798720 @default.
• W3197894855 cites W1972566315 @default.
• W3197894855 cites W1976599392 @default.
• W3197894855 cites W1976891945 @default.
• W3197894855 cites W1980073547 @default.
• W3197894855 cites W1984247046 @default.
• W3197894855 cites W1990873429 @default.
• W3197894855 cites W1990900785 @default.
• W3197894855 cites W1997161797 @default.
• W3197894855 cites W1997480152 @default.
• W3197894855 cites W1998049513 @default.
• W3197894855 cites W2017356951 @default.
• W3197894855 cites W2018003660 @default.
• W3197894855 cites W2019979171 @default.
• W3197894855 cites W2022070994 @default.
• W3197894855 cites W2030695915 @default.
• W3197894855 cites W2036127346 @default.
• W3197894855 cites W2037427470 @default.
• W3197894855 cites W2039210066 @default.
• W3197894855 cites W2052426129 @default.
• W3197894855 cites W2054129051 @default.
• W3197894855 cites W2060053190 @default.
• W3197894855 cites W2064388791 @default.
• W3197894855 cites W2066706135 @default.
• W3197894855 cites W2069054307 @default.
• W3197894855 cites W2069271483 @default.
• W3197894855 cites W2080791625 @default.
• W3197894855 cites W2082335522 @default.
• W3197894855 cites W2085849897 @default.
• W3197894855 cites W2088256976 @default.
• W3197894855 cites W2090212447 @default.
• W3197894855 cites W2109026370 @default.
• W3197894855 cites W2120599576 @default.
• W3197894855 cites W2126102214 @default.
• W3197894855 cites W2128172592 @default.
• W3197894855 cites W2134147483 @default.
• W3197894855 cites W2148760231 @default.
• W3197894855 cites W2151228364 @default.
• W3197894855 cites W2160071206 @default.
• W3197894855 cites W2161996750 @default.
• W3197894855 cites W2336548300 @default.
• W3197894855 cites W2518896446 @default.
• W3197894855 cites W2565319794 @default.
• W3197894855 cites W2932695908 @default.
• W3197894855 cites W2935583638 @default.
• W3197894855 cites W2952138778 @default.
• W3197894855 cites W3037629148 @default.
• W3197894855 cites W3109926661 @default.
• W3197894855 cites W3113291359 @default.
• W3197894855 doi "https://doi.org/10.1016/j.jbc.2021.101186" @default.
• W3197894855 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8487062" @default.
• W3197894855 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34517006" @default.
• W3197894855 hasPublicationYear "2021" @default.
• W3197894855 type Work @default.
• W3197894855 sameAs 3197894855 @default.
• W3197894855 citedByCount "0" @default.
• W3197894855 crossrefType "journal-article" @default.
• W3197894855 hasAuthorship W3197894855A5001152422 @default.
• W3197894855 hasAuthorship W3197894855A5021865068 @default.
• W3197894855 hasAuthorship W3197894855A5040684273 @default.
• W3197894855 hasAuthorship W3197894855A5049221447 @default.
• W3197894855 hasAuthorship W3197894855A5055601896 @default.
• W3197894855 hasAuthorship W3197894855A5081980355 @default.
• W3197894855 hasBestOaLocation W31978948551 @default.
• W3197894855 hasConcept C104317684 @default.
• W3197894855 hasConcept C121332964 @default.
• W3197894855 hasConcept C12554922 @default.
• W3197894855 hasConcept C142613039 @default.
• W3197894855 hasConcept C178790620 @default.
• W3197894855 hasConcept C183688256 @default.
• W3197894855 hasConcept C185592680 @default.
• W3197894855 hasConcept C192468462 @default.
• W3197894855 hasConcept C2777105759 @default.
• W3197894855 hasConcept C2777267445 @default.
• W3197894855 hasConcept C2780542282 @default.
• W3197894855 hasConcept C5176359 @default.
• W3197894855 hasConcept C55493867 @default.
• W3197894855 hasConcept C55904794 @default.
• W3197894855 hasConcept C62520636 @default.
• W3197894855 hasConcept C75473681 @default.
• W3197894855 hasConcept C80298142 @default.

• next