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- W3197906068 abstract "Abstract Architectural proteins alter the shape of DNA. Some distort the double helix by introducing sharp kinks. This can serve to relieve strain in tightly-bent DNA structures. Here, we design and test artificial architectural proteins based on a sequence-specific Transcription Activator-like Effector (TALE) protein, either alone or fused to a eukaryotic high mobility group B (HMGB) DNA-bending domain. We hypothesized that TALE protein binding would stiffen DNA to bending and twisting, acting as an architectural protein that antagonizes the formation of small DNA loops. In contrast, fusion to an HMGB domain was hypothesized to generate a targeted DNA-bending architectural protein that facilitates DNA looping. We provide evidence from Escherichia coli Lac repressor gene regulatory loops supporting these hypotheses in living bacteria. Both data fitting to a thermodynamic DNA looping model and sophisticated molecular modeling support the interpretation of these results. We find that TALE protein binding inhibits looping by stiffening DNA to bending and twisting, while the Nhp6A domain enhances looping by bending DNA without introducing twisting flexibility. Our work illustrates artificial approaches to sculpt DNA geometry with functional consequences. Similar approaches may be applicable to tune the stability of small DNA loops in eukaryotes." @default.
- W3197906068 created "2021-09-13" @default.
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- W3197906068 date "2021-09-03" @default.
- W3197906068 modified "2023-10-17" @default.
- W3197906068 title "Designed architectural proteins that tune DNA looping in bacteria" @default.
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- W3197906068 cites W2001857107 @default.
- W3197906068 cites W2002767268 @default.
- W3197906068 cites W2002902680 @default.
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- W3197906068 cites W2101636425 @default.
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- W3197906068 cites W2104819112 @default.
- W3197906068 cites W2114163400 @default.
- W3197906068 cites W2123911104 @default.
- W3197906068 cites W2126085819 @default.
- W3197906068 cites W2130931930 @default.
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- W3197906068 cites W2790694026 @default.
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- W3197906068 doi "https://doi.org/10.1093/nar/gkab759" @default.
- W3197906068 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8501960" @default.
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- W3197906068 hasPublicationYear "2021" @default.
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