FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3197989845> ?p ?o ?g. }

• W3197989845 endingPage "4938" @default.
• W3197989845 startingPage "4938" @default.
• W3197989845 abstract "We thank Kossatz and Notni for their interest in our article about the use of fluorescent cRGD-ZW800-1 for visualization of tumor tissue in humans during surgery (1). Their concern seems the specificity of the tracer for integrin αvβ6, which is lower than for αvβ3, and the relation with our hypothesis. The latter seems clearly defined: “Documentation of the first clinical experience with cRGD-ZW800-1, particularly in patients with colonic cancer.” The specificity of the tracer for αvβ6 was clearly of secondary importance, indicated by the absence in title and abstract. Furthermore, we have stated that the cRGD peptide is applicable for many integrins: “cRGD is a clinically well-known peptide that binds to a wide range of integrins, including αvβ1, αvβ3, αvβ5, αvβ6, and αvb8.”Concerning the affinity of cRGD-ZW800-1: in our (pre)clinical experience, there is no “threshold” affinity for effective in vivo imaging in the complex microenvironment of a malignant tumor. Many variables contribute to a tumor-to-background ratio of fluorescent signal, including number of targets on the cells, ligand on/off-rate, ligand-induced endocytosis, presence of various cell types, and intratumoral pressure. The Kapp paper mentions that the affinity (the ratio of off-rate to on-rate) of our cRGDyK sequence for αvβ6 is 86 nmol/L (2), a low but respectable affinity for effective in vivo imaging, especially for target concentrations. Suppose that the αvβ6 copy number on malignant cells is 10-fold higher than for αvβ3, but the cRGD affinity is 10-fold lower; the resulting net moles of ligand-bound-per-cell would be exactly the same! We previously showed that cells without detectable αvβ3 on their membrane are perfectly visualized by cRGD-ZW800-1, due to the presence of other integrins with relatively low cRGD affinity (3). Regarding the authors' remark: The statement “Uptake of cRGD-ZW800–1 by αvβ6-integrin expressing HT29 cells could not be blocked by competition (no significance)” is incorrect, referring to Fig. 1C where an in vivo competition experiment performed using HT-29 cells was statistically significant to P = 0.02 and P = 007, at, respectively, 4 and 24 hours after injection. The in vitro experiment had the identical trend, although not statistically significant.Our clinically oriented paper deliberately focused on the correlation of αvβ6 expression and cRGD-ZW800–1 signal strength, because αvβ3 has already been discussed ad nauseum. And indeed, the correlation existed, regardless whether it was dependent of αvβ3 coexpression or not. In terms of integrin biology and clinical perspective, the most important finding was the strong αvβ6 expression in positive lymph nodes, but absence in metastasis free nodes. This could be of key importance for clinical application. Therefore, we are eager to discuss and learn from experts of various disciplines to optimize our materials or models, but in the end the proof of the pudding is in the eating, established in the clinic.See the original Letter to the Editor, p. 4937No disclosures were reported." @default.
• W3197989845 created "2021-09-13" @default.
• W3197989845 creator A5020233877 @default.
• W3197989845 creator A5028075025 @default.
• W3197989845 date "2021-09-01" @default.
• W3197989845 modified "2023-10-17" @default.
• W3197989845 title "NIR Fluorescence Imaging of Colon Cancer With cRGD-ZW800-1—Response" @default.
• W3197989845 cites W2578530566 @default.
• W3197989845 cites W2589907317 @default.
• W3197989845 cites W3020160908 @default.
• W3197989845 doi "https://doi.org/10.1158/1078-0432.ccr-21-1354" @default.
• W3197989845 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34470809" @default.
• W3197989845 hasPublicationYear "2021" @default.
• W3197989845 type Work @default.
• W3197989845 sameAs 3197989845 @default.
• W3197989845 citedByCount "0" @default.
• W3197989845 crossrefType "journal-article" @default.
• W3197989845 hasAuthorship W3197989845A5020233877 @default.
• W3197989845 hasAuthorship W3197989845A5028075025 @default.
• W3197989845 hasBestOaLocation W31979898451 @default.
• W3197989845 hasConcept C116569031 @default.
• W3197989845 hasConcept C121332964 @default.
• W3197989845 hasConcept C121608353 @default.
• W3197989845 hasConcept C12554922 @default.
• W3197989845 hasConcept C126322002 @default.
• W3197989845 hasConcept C142724271 @default.
• W3197989845 hasConcept C1491633281 @default.
• W3197989845 hasConcept C150903083 @default.
• W3197989845 hasConcept C153911025 @default.
• W3197989845 hasConcept C170493617 @default.
• W3197989845 hasConcept C185592680 @default.
• W3197989845 hasConcept C195687474 @default.
• W3197989845 hasConcept C207001950 @default.
• W3197989845 hasConcept C28005876 @default.
• W3197989845 hasConcept C502942594 @default.
• W3197989845 hasConcept C526805850 @default.
• W3197989845 hasConcept C55493867 @default.
• W3197989845 hasConcept C62520636 @default.
• W3197989845 hasConcept C71924100 @default.
• W3197989845 hasConcept C86803240 @default.
• W3197989845 hasConcept C91881484 @default.
• W3197989845 hasConceptScore W3197989845C116569031 @default.
• W3197989845 hasConceptScore W3197989845C121332964 @default.
• W3197989845 hasConceptScore W3197989845C121608353 @default.
• W3197989845 hasConceptScore W3197989845C12554922 @default.
• W3197989845 hasConceptScore W3197989845C126322002 @default.
• W3197989845 hasConceptScore W3197989845C142724271 @default.
• W3197989845 hasConceptScore W3197989845C1491633281 @default.
• W3197989845 hasConceptScore W3197989845C150903083 @default.
• W3197989845 hasConceptScore W3197989845C153911025 @default.
• W3197989845 hasConceptScore W3197989845C170493617 @default.
• W3197989845 hasConceptScore W3197989845C185592680 @default.
• W3197989845 hasConceptScore W3197989845C195687474 @default.
• W3197989845 hasConceptScore W3197989845C207001950 @default.
• W3197989845 hasConceptScore W3197989845C28005876 @default.
• W3197989845 hasConceptScore W3197989845C502942594 @default.
• W3197989845 hasConceptScore W3197989845C526805850 @default.
• W3197989845 hasConceptScore W3197989845C55493867 @default.
• W3197989845 hasConceptScore W3197989845C62520636 @default.
• W3197989845 hasConceptScore W3197989845C71924100 @default.
• W3197989845 hasConceptScore W3197989845C86803240 @default.
• W3197989845 hasConceptScore W3197989845C91881484 @default.
• W3197989845 hasIssue "17" @default.
• W3197989845 hasLocation W31979898451 @default.
• W3197989845 hasLocation W31979898452 @default.
• W3197989845 hasOpenAccess W3197989845 @default.
• W3197989845 hasPrimaryLocation W31979898451 @default.
• W3197989845 hasRelatedWork W1980377213 @default.
• W3197989845 hasRelatedWork W1990859943 @default.
• W3197989845 hasRelatedWork W2050345288 @default.
• W3197989845 hasRelatedWork W2050940716 @default.
• W3197989845 hasRelatedWork W2098438673 @default.
• W3197989845 hasRelatedWork W2167884612 @default.
• W3197989845 hasRelatedWork W2198850752 @default.
• W3197989845 hasRelatedWork W2345755371 @default.
• W3197989845 hasRelatedWork W2606830224 @default.
• W3197989845 hasRelatedWork W2910354686 @default.
• W3197989845 hasVolume "27" @default.
• W3197989845 isParatext "false" @default.
• W3197989845 isRetracted "false" @default.
• W3197989845 magId "3197989845" @default.
• W3197989845 workType "article" @default.